Tuberculosis is one of the most persistent and deadliest
infectious diseases in the world, killing one to two million people
each year.
Scientists who study tuberculosis have long debated its origins.
New research shows that tuberculosis likely spread from humans in
Africa to seals and sea lions that brought the disease to South
America and transmitted it to Native people there before Europeans
landed on the continent.
"We found that the tuberculosis strains were most closely
related to strains in pinnipeds, which are seals and sea lions,"
said co-lead researcher Professor Anne Stone of Arizona State
University. "What we found was really surprising. The ancient
strains are distinct from any known human-adapted tuberculosis
strain."
Professor Johannes Krause of the University of Tubingen in
Germany was the other co-lead investigator on the project, and
worked alongside collaborators from the Wellcome Trust Sanger
Institute and the Swiss Institute for Tropical and Public Health in
Basel. Professor Douglas Young of the Medical Research Council's
National Institute for Medical Research (now part of the
Francis Crick Institute) and Imperial College London
also made a contribution to the work.
Modern strains of tuberculosis currently circulating are most
closely related to those found in Europe, and there was a complete
replacement of the older strains when European disease reached the
Americas during the age of exploration. Researchers found that
genomes from humans in Peru dating from about 1,000 year ago
provide unequivocal evidence that a member of the tuberculosis
strain caused disease in South America before Europeans arrived, so
the question among the scientists was, "What types of tuberculosis
strains were present before contact?"
"The age of exploration is a time when people are moving really
long distances around the world and coming into contact with
others. It's a time when a lot of disease spread," Professor Stone
said. "This opens up a lot of new questions. It fits the
bioarcheological evidence that shows the oldest evidence for
tuberculosis in South America."
Professor Krause added: "The connection to seals and sea lions
is important to explain how a mammalian-adapted pathogen that
evolved in Africa around 6,000 years ago could have reached Peru
5,000 years later."
In the study, researchers collected genetic samples from
throughout the world and tested those for tuberculosis DNA while
utilising advances in technology during the past five years that
enable more accurate genome capture from ancient samples. Of 76 DNA
samples from New World pre- and post-contact sites, three from Peru
around 750 to 1350 AD had tuberculosis DNA that could be used. The
researchers then focused on these three samples and used
array-based capture to obtain and map the complete genome.
These were compared against a larger dataset of modern genomes
and animal strains. Research results showed the clear relationship
to animal lineages, specifically seals and sea lions.
"Our results show unequivocal evidence of human infection caused
by pinnipeds (sea lions and seals) in pre-Columbian South America.
Within the past 2,500 years, the marine animals likely contracted
the disease from an African host species and carried it across the
ocean to coastal people in South America," Professor Stone
said.
Africa has the most diversity among tuberculosis strains,
implying that the pathogen likely originated from the continent and
spread. After tuberculosis was established in South America, it may
have moved north and infected people in North America before
European settlers brought new strains in.
"We hypothesise that when the more virulent European strains
came, they quickly replaced the pinniped strains," said Professor
Stone.
Study implications include a greater understanding of the speed
and process of adaptation when a disease changes hosts. This is
especially of interest when considering diseases that are
transmitted between species - MERS, SARS and HIV - and how these
are spread, she added.
The paper, Pre-Columbian Mycobacterial Genomes Reveal Seals as a Source of New
World Human Tuberculosis, is published inNature.