Senior Laboratory Research Scientist

In the Crick's Stp |Proteomics Team.

Applies an in-depth knowledge of research, technical or other scientific practices, methods and procedures gained through either experience and/or formal academic qualifications and applies this knowledge to provide a range of specialist activities. Senior Laboratory Research Scientist is the level at which careers in this functional family typically stabilise.

Key information

Job reference
R132
Salary
Competitive with benefits, subject to skills and experience.
Application close date
29 May 2021, 00:00 BST
Hours per week
36 (full time)
Posted 30 April 2021

Senior Laboratory Research Scientist

Proteomics STP

Closing date: 28th May 2021 23:59

Contract term: This is a permanent full-time position on Crick Terms and Conditions of Employment

Project Summary

We are looking for an enthusiastic and experienced proteomics specialist at Senior Laboratory Research Scientist (SLRS) level to complement our team at the Francis Crick Institute in the Proteomics Scientific Technology Platform (STP).  The post holder will help us provide high-level service and contribute towards key research with Crick scientists. They will be responsible for providing support in all aspects of STP work, from experimental design and sample preparation to data acquisition and analysis.

The STP houses a broad range of state-of-the-art mass spectrometry equipment including (but not limited to) a Thermo Scientific Orbitrap Eclipse FAIMS ETD, a Bruker timsTOF Pro, a Waters SYNAPT HDX platform and Thermo Scientific QEUHMR.  The role will involve setting up new experiments as projects arise using the available mass spectrometry technologies to both qualitatively and quantitatively measure proteins. The successful candidate will be able to take initiative in project design and interact closely with collaborators in order to deliver results efficiently.  To be considered for this role the applicant must have >3 years’ hands-on experience of working in a protein/peptide mass spectrometry laboratory.

Key responsibilities

These include but are not limited to:

  • Planning and designing experimental workflows for protein and peptide analysis.

  • Performing both routine and bespoke proteomics experiments starting with sample preparation from a variety of biological sources and using methodology such as cell lysis, running and staining SDS-PAGE gels, in-gel, in-solution, TCA and acetone precipitation digest protocols, desalting of peptide samples, TMT labelling, HpH fractionation.

  • LC-MS/MS nanoUHPLC data acquisition and evaluation and processing of raw data, including QC monitoring.

Key experience and competencies

The post holder should embody and demonstrate our core Crick values: bold, imaginative, open, dynamic and collegial, in addition to the following:

Essential

Qualifications, experience and competencies:

  • A PhD or equivalent experience gained through working in a mass spectrometry laboratory, including basic and advanced proteomic sample handling and processing protocols, and familiarity with instrument maintenance requirements.

  • >3 years’ experience in proteomics mass spectrometry

  • Direct experience with either the Thermo Scientific Orbitrap Tribrid platform or the Bruker timsTOF Pro.

  • Familiarity with data analysis software such as Proteome Discoverer, PEAKS, MaxQuant, Spectronaut and Skyline.

  • Experience of independently organising and running a project.

  • Excellent communication, record keeping, time management and problem solving skills

  • Ability to work confidently and independently.

Desirable

Qualifications, experience and competencies:

  • Experience working in a customer-facing service laboratory (academia or industry).

  • Set up and operation of automation and liquid handling for high throughput sample preparation techniques.

  • Conducting experiments with MS cleavable crosslinkers (DSSO, DSBU) from wet lab through to data analysis.

  • Experience with maintaining nanoLC, Orbitrap or Q-TOF equipment.

  • Familiarity with a range of advanced proteomic workflows and techniques (SILAC, TMT, DIA, PTM enrichment etc).

  • Publication record demonstrating contribution using proteomics and mass spectrometry applications or methodology.