Senior/Laboratory Research Scientist
In the Crick's Lee J |Research Group.
Key information
Senior/Laboratory Research Scientist
Reports to: Dr James Lee, Clinician Scientist Group Leader
Closing Date: Thursday 20th May, 2021
This is a full-time, permanent position on Crick terms and conditions of employment.
Project Summary
We are looking for an experienced researcher with a broad range of laboratory, bioinformatic and administrative skills. The candidate will be expected to co-ordinate laboratory management and associated administrative tasks, and will also actively participate in the design and analysis of experiments using primary immune cells from humans and mice (including NGS). Extensive experience in molecular biology and in working with human immune cells is a pre-requisite, as is experience in conducting and analysing RNA-seq, ChIP-seq and MPRA data.
Our lab is moving from Cambridge to the Crick in July 2021 and will focus on resolving the molecular, cellular and immunological mechanisms by which genetic variation contributes to the pathogenesis of autoimmune and inflammatory disease.
Key responsibilities
These include but are not limited to:
- Lab management, including organising lab space, ordering consumables, supporting students and grant management
- Designing and conducting experiments using primary human immune cells, including genome editing
- Providing bioinformatic support for NGS-based data analysis
- Mouse colony management and contributing to experiments to study immune responses
Key experience and competencies
The post holder should embody and demonstrate our core Crick values: bold, imaginative, open, dynamic and collegial, in addition to the following:
Essential
Qualifications, experience and competencies:
- Experience with isolation, culture and transfection of primary human T cells and macrophages
- Experience with RNA-sequencing, ChIP-sequencing, and CUT&RUN (including experimentation, library prep and data pre-processing and analysis)
- Experience with MPRA in non-cell lines, including vector design and cloning
- Experience with multicolour flow cytometry, including panel design
- PIL holder with experience in colony management and colitis models
- Experience in lab management, including supporting students, grant management, risk assessment and writing SOPs
- Master’s degree (or equivalent)
Desirable
Qualifications, experience and competencies:
- Experience with nascent RNA capture methods
- Experience with assays of immune function, including phagocytosis, reactive oxygen species production and cellular polarisation
- Experience of antibiotic-free cell culture
