Reports to: Alberto Elosegui-Artola, Group Leader
This is a full-time, fixed term (12 months with potential renewal subject to funding) position on Crick terms and conditions of employment, which could also be offered as a secondment.
The Cell and Tissue Mechanobiology lab led by Alberto Elosegui-Artola is seeking a Senior Laboratory research scientist (SLRS) to support ongoing projects in the lab as well as lead independent research. The post holder is expected to develop tools and technologies, manage day-to-day lab operations and train PhDs and postdocs. The suitable candidate will study how the interplay between mechanics and chemical cues regulate tissues response in a multidisciplinary environment. The candidate will work with 3D organotypic systems, use advanced microscopic techniques/image analysis, generate transgenic lines/CRISPR mutants/knock-ins, use biophysical techniques and genetic manipulations to understand the fundamentals of cell and tissue mechanobiology in 3D. The post holder should be strongly motivated to lead independent research projects as well as support lab’s ongoing projects and should be willing to collaborate within and outside the lab.
We are looking for researchers interested in a multidisciplinary approach to determine the interplay between mechanical and chemical cues in living tissues. We utilize a variety of cellular systems like 3D organotypic systems, organoids and primary cells, combined with 3D matrix engineering and microfluidics coupled to advanced microscopy to gain a fundamental understanding of the role of mechanics in tissue behavior. Some of the specific aims are: to study the interaction between tumors and the stroma, to analyze the role of the extracellular matrix in organoid morphogenesis in health and disease; to unravel the mechanical and molecular mechanisms that cells utilize to sense the 3D extracellular matrix; to guide tissue response through mechanical cues.
We and others have shown that cells and tissues sense and respond to different mechanical stimuli both in 2D and 3D and that regulates different signalling pathways (Elosegui-Artola et al., Nat. Mater. 2014; Elosegui-Artola et al., Nat. Cel. Biol 2016; Elosegui-Artola et al., Cell 2017; Elosegui-Artola, COCB 2021). The goal of the lab is to understand how physical properties and biochemical cues work together to regulate biological functions. We want to understand how the interplay between physical properties and biochemical stimuli regulate biological function at the molecular, cellular and tissue level. We study these questions by studying the interaction between cells and the extracellular matrix to determine the mechanisms that cells use to sense and respond to mechanical stimuli. We study how these mechanisms are regulated in healthy tissue and during development, and how they can be derailed in diseases like cancer. We combine molecular biology, microscopy with biophysics and bioengineering to address these questions.
Members of the lab benefit from access to phenomenal scientific technology platforms of the Francis Crick Institute and its collaborative environment alongside other research groups. We have strong core funding and we have been awarded an ERC Starting Grant.
Key experience and competencies
The post holder should embody and demonstrate our core Crick values: bold, imaginative, open, dynamic and collegial, in addition to the following:
- Higher education degree in biology, bioengineering or a related field.
- Strong expertise in state-of-the-art molecular biology and genetics in mammalian cell biology.
- Track record of writing papers as evidenced by publications in referred journals/preprints.
- Track record of working on collaborative projects and teamwork experience.
- Strong interest in pursuing independent research.
- Experience in advanced microscopy imaging and image analysis.
- Previous work in tumour or developmental biology.
- Previous experience in in-vivo experiment.
- PhD in biology, bioengineering or a related field.
- Previous work with 3D organotypic models or with organoids.
- Experience in biophysics/mechanobiology at the cell or tissue level.