Senior Laboratory Research Scientist - Proteomics

In the Crick's Stp |Proteomics Team.

Applies an in-depth knowledge of research, technical or other scientific practices, methods and procedures gained through either experience and/or formal academic qualifications and applies this knowledge to provide a range of specialist activities. Senior Laboratory Research Scientist is the level at which careers in this functional family typically stabilise.
Deadline for applications has passed.

Key information

Job reference
R724
Salary
From £36,500 with benefits, subject to skills and experience.
Applications closed
22 January 2023, 23:59 GMT
Hours per week
36 (full time)
Posted 16 December 2022

Senior Laboratory Research Scientist Proteomics STP

This is a permanent position on Crick Terms and Conditions of Employment.

Summary

Are you passionate about biological mass spectrometry and applying state-of-the-art proteomics technologies?

We have an exciting opportunity for a talented, experienced, and motivated Senior Laboratory Research Scientist in the Proteomics Scientific Technology Platform (STP) at the Francis Crick Institute. The post holder will help us provide a world class platform to contribute towards key research in collaboration with the Crick scientific community.  This is a lab-based position, responsible for driving forward projects, from experimental design through sample preparation to data analysis.

The Proteomics STP houses a broad range of mass spectrometers including a Thermo Scientific Orbitrap Eclipse Tribrid with FAIMS and ETD, Exploris 480, Q Exactive UHMR, Bruker timsTOF Pro, Waters Select Cyclic and SYNAPT G2Si HDMS.  The role will involve setting up new experiments as projects arise using the available mass spectrometry technologies to both qualitatively and quantitatively measure proteins. The successful candidate will be able to take initiative in project design and interact closely with collaborators. It is also anticipated that the successful candidate will bring on board and apply cutting-edge proteomics methods such “Single Cell Proteomics” applying the approach to discovery and translational research at the Crick. To be considered for this role the applicant must have >3 years hands-on experience of working in a proteomics mass spectrometry laboratory.

Key responsibilities

These include but are not limited to:

  • Planning of experiments, decision making about which pipeline to take, and independently executing experiments for projects.

  • Performing both standard and bespoke proteomics experiments starting with sample preparation from a variety of biological sources using common biochemical methodologies for cell lysis, SDS-PAGE, in-gel, in-solution, TCA and acetone precipitation digest protocols, TMT labelling and High pH chromatographic fractionation.

  • LC-MS/MS nanoUHPLC data acquisition, evaluation, and processing of raw data.

  • Communication of results to collaborators.

Key experience and competencies

The post holder should embody and demonstrate our core Crick values: bold, imaginative, open, dynamic and collegial, in addition to the following:

Essential

Qualifications, experience and competencies:

  • A PhD or equivalent experience gained through working in a mass spectrometry laboratory, including basic and advanced proteomic sample handling and processing protocols, and familiarity with instrument maintenance requirements.

  • Direct experience with either the Thermo Scientific Orbitrap Tribrid platform or the Bruker timsTOF Pro.

  • Detailed understanding of proteomics related bioinformatics and associated software packages such as Proteome Discoverer, PEAKS, Mascot, Scaffold, MaxQuant, Spectronaut and Skyline.

  • Experience of independently organising and running projects.

  • Excellent communication, record keeping, time management and problem-solving skills

  • Ability to work confidently and independently, approaching research problems with initiative and flexibility.

Desirable

Qualifications, experience and competencies:

  • Experience working in a customer-facing laboratory (academia or industry).

  • Set up and operation of automation and liquid handling for high throughput sample preparation techniques.

  • Experience with proteome level post-translational modification analyses (e.g., phosphorylation, ubiquitination, acetylation/methylation).

  • Experience with maintaining nanoLC, Orbitrap or Q-TOF instrumentation.

  • Experience with developing, optimizing and applying next-generation proteomics and mass spectrometry methods such as DIA/SWATH, DDA, label free profiling, TMT, PRM etc.

  • Basic understanding of biostatistics and computational programming.

  • Publication record demonstrating contribution using proteomics and mass spectrometry applications or methodology.

For informal queries on the role please contact Dr J. Mark Skehel by email: skehelm@crick.ac.uk