John Diffley: Projects

Future projects

We have reconstituted the initiation of DNA replication with purified proteins. We will use this system to understand how the MCM replicative helicase is activated and how this leads to assembly of the replisome. We will also use this system to understand how DNA replication interfaces with chromatin assembly, RNA transcription and many other nuclear processes.

We use a variety of mammalian models to understand how the deregulation of normal replication control mechanisms leads to genomic instability and whether this plays a role in cancer biology.

Figure 1

Figure 1: A simplified view of eukaryotic DNA replication origin firing, with an emphasis on current interests of the Diffley Lab.


John Diffley
+44 (0)379 61833

  • Qualifications and history
  • 1985 PhD, New York University, USA
  • 1984 Postdoctoral Fellow, Cold Spring Harbor Laboratory, USA
  • 1990 Established lab at the Imperial Cancer Research Fund, UK (in 2002 the Imperial Cancer Research Fund became Cancer Research UK)
  • 2015 Associate Research Director, the Francis Crick Institute, London, UK