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MCM helicase loaded onto duplex DNA Toggle to view hero caption Alessandro Costa : Publications Lab MenuLab Menu Overview Areas of interest View more Back The replicative helicase The replisome Chromatin replication Publications Members Vacancies Breadcrumb Home Research Labs Alessandro Costa Publications PublicationsYear published2020 (3)2019 (4)2018 (3)2017 (10)2016 (4)2015 (4)2014 (4)2013 (3)2012 (1)Research topicsBiochemistry & Proteomics (26)Cell Cycle & Chromosomes (26)Genome Integrity & Repair (26)Imaging (26)Structural Biology & Biophysics (26)Model Organisms (9)Genetics & Genomics (8)Cell Biology (7)Chemical Biology & High Throughput (5)Computational & Systems Biology (4)Infectious Disease (2)Synthetic Biology (1)Publication typeJournal article (36)Primary research (24)Review (8)Commentary (3)Method (1)JournalNature Communications (5)Nature (4)Proceedings of the National Academy of Sciences of the United States of America (4)Cell Reports (3)Structure (3)eLife (2)Molecular Cell (2)Acta crystallographica. Section D, Structural biology (1)Annual Review of Biochemistry (1)Biochemical Society Transactions (1)Biophysical Chemistry (1)Cell & Bioscience (1)Chromosoma (1)Current Opinion in Structural Biology (1)Developmental Cell (1)DNA Repair (1)Journal of Molecular Biology (1)Methods in Molecular Biology (1)Science (1)Trends in Biochemical Sciences (1) Publications Displaying 1 - 20 of 36Last updated : 11 July 2020 02:21 Microtubule nucleation properties of single human γTuRCs explained by their cryo-EM structure Authors (12)Tanja ConsolatiJulia LockeJohanna Roostalu Zhuo Angel ChenJulian Gannon Jayant Asthana[...]Fabrizio MartinoMilos Cvetkovic Juri RappsilberAlessandro CostaThomas Surrey Developmental Cell Published 8 June 2020 Crick labs/ facilitiesAlessandro CostaThomas SurreyCell ServicesElectron MicroscopyFlow CytometryProteomicsStructural BiologyFull details Caught in the act: structural dynamics of replication origin activation and fork progression Authors (2) Jacob S LewisAlessandro Costa Biochemical Society Transactions Published 5 May 2020 Crick labsAlessandro CostaFull details A different twist on centromeric chromatin Authors (2) Fabrizio MartinoAlessandro Costa Structure Published 7 January 2020 Crick labsAlessandro CostaFull details Mechanism of head-to-head MCM double-hexamer formation revealed by cryo-EM Authors (5)Thomas MillerJulia LockeJulia GreiweJohn DiffleyAlessandro Costa Nature Published 20 November 2019 Crick labs/ facilitiesAlessandro CostaJohn DiffleyElectron MicroscopyStructural BiologyFermentationFull details A combined structural and biochemical approach reveals translocation and stalling of UvrB on the DNA lesion as a mechanism of damage verification in bacterial nucleotide excision repair Authors (10) Marcin Jaciuk Paolo Swuec Vineet Gaur Joanna M Kasprzak Ludovic Renault Mateusz Dobrychłop Shivlee Nirwal Janusz M BujnickiAlessandro Costa Marcin Nowotny DNA Repair Published 1 January 2020 Crick labsAlessandro CostaFull details Retroviral integration into nucleosomes through DNA looping and sliding along the histone octamer Authors (9) Marcus D Wilson Ludovic Renault Daniel P Maskell Mohamed GhoneimValerie PyeAndrea Nans David S RuedaPeter CherepanovAlessandro Costa Nature Communications Published 13 September 2019 Crick labs/ facilitiesPeter Cherepanov Alessandro CostaStructural BiologyElectron MicroscopyFull details Molecular basis for ATP-hydrolysis-driven DNA translocation by the CMG helicase of the eukaryotic replisome Authors (12)Patrik EickhoffHazal KoseFabrizio Martino Tatjana Petojevic Ferdos Abid AliJulia Locke[...]Andrea Nans James M Berger Michael R BotchanHasan YardimciAlessandro Costa Cell Reports Published 3 September 2019 Crick labs/ facilitiesAlessandro CostaHasan YardimciStructural BiologyElectron MicroscopyFull details Structure of DNA-CMG-Pol epsilon elucidates the roles of the non-catalytic polymerase modules in the eukaryotic replisome Authors (12) Panchali Goswami Ferdos Abid Ali Max E Douglas Julia Locke Andrew Purkiss Agnieszka Janska[...] Anne EarlyAndrea Nans Alan MC CheungJohn DiffleyAlessandro Costa Nature Communications Published 29 November 2018 Crick labs/ facilitiesAlessandro CostaJohn DiffleyStructural BiologyElectron MicroscopyFull details Preparing frozen-hydrated protein-nucleic acid assemblies for high-resolution cryo-EM imaging Authors (3) Panchali Goswami Julia LockeAlessandro Costa Methods in Molecular Biology Published 30 June 2018 Crick labsAlessandro CostaFull details The mechanism of eukaryotic CMG helicase activation Authors (4) Max E Douglas Ferdos Abid AliAlessandro CostaJohn Diffley Nature Published 8 March 2018 Crick labs/ facilitiesAlessandro CostaJohn DiffleyBioinformatics and BiostatisticsElectron MicroscopyFull details Cryo-EM structure of a licensed DNA replication origin Authors (7) Ferdos Abid Ali Max E Douglas Julia Locke Valerie E PyeAndrea NansJohn DiffleyAlessandro Costa Nature Communications Published 21 December 2017 Crick labs/ facilitiesPeter Cherepanov Alessandro CostaJohn DiffleyPeter RosenthalElectron MicroscopyStructural BiologyFull details Mechanism of ubiquitination and deubiquitination in the Fanconi anemia pathway Authors (9) Sylvie van Twest Vincent J Murphy Charlotte Hodson Winnie Tan Paolo Swuec Julienne J O'Rourke Jörg Heierhorst Wayne Crismani Andrew J Deans Molecular Cell Published 9 December 2017 Crick labsAlessandro CostaFull details The FA core complex contains a homo-dimeric catalytic module for the symmetric mono-ubiquitination of FANCI-FANCD2 Authors (9) Paolo Swuec Ludovic Renault Aaron Borg Fenil Shah Vincent J Murphy Sylvie van TwestBram Snijders Andrew J DeansAlessandro Costa Cell Reports Published 9 December 2017 Crick labs/ facilitiesAlessandro CostaProteomicsFull details DNA replication and inter-strand crosslink repair: Symmetric activation of dimeric nanomachines? Authors (2) Paolo SwuecAlessandro Costa Biophysical Chemistry Published 10 November 2017 Crick labsAlessandro CostaFull details Escorting client proteins to the Hsp90 molecular chaperone Authors (2)Patrik EickhoffAlessandro Costa Structure Published 5 July 2017 Crick labsAlessandro CostaFull details Cdt1 stabilizes an open MCM ring for helicase loading Authors (10) Jordi Frigola Jun He Kerstin Kinkelin Valerie E Pye Ludovic Renault Max E Douglas Dirk RemusPeter CherepanovAlessandro CostaJohn Diffley Nature Communications Published 23 June 2017 Crick labsPeter Cherepanov Alessandro CostaJohn DiffleyFull details Cryo-electron microscopy of chromatin biology Authors (2) Marcus D WilsonAlessandro Costa Acta crystallographica. Section D, Structural biology Published 1 June 2017 Crick labsAlessandro CostaFull details The architecture and function of the chromatin replication machinery Authors (2)Thomas MillerAlessandro Costa Current Opinion in Structural Biology Published 15 April 2017 Crick labsAlessandro CostaFull details CMG-Pol epsilon dynamics suggests a mechanism for the establishment of leading-strand synthesis in the eukaryotic replisome Authors (8) Jin Chuan Zhou Agnieszka Janska Panchali Goswami Ludovic Renault Ferdos Abid Ali Abhay KotechaJohn DiffleyAlessandro Costa Proceedings of the National Academy of Sciences of the United States of America Published 3 April 2017 Crick labsAlessandro CostaJohn DiffleyFull details A supramolecular assembly mediates lentiviral DNA integration Authors (14)Allison Ballandras-Colas Daniel P Maskell Erik SerraoJulia Locke Paolo Swuec Stefán R Jónsson[...]Ian Taylor Valgerdur Andrésdóttir Alan N EngelmanAlessandro CostaPeter Cherepanov Science Published 6 January 2017 Crick labs/ facilitiesPeter Cherepanov Alessandro CostaIan TaylorElectron MicroscopyStructural BiologyFull details Pagination Current page 1 Page 2 Next page Next Last page Last Share the page Share on Twitter Share on Facebook Share on LinkedIn Share on Email
Microtubule nucleation properties of single human γTuRCs explained by their cryo-EM structure Authors (12)Tanja ConsolatiJulia LockeJohanna Roostalu Zhuo Angel ChenJulian Gannon Jayant Asthana[...]Fabrizio MartinoMilos Cvetkovic Juri RappsilberAlessandro CostaThomas Surrey Developmental Cell Published 8 June 2020 Crick labs/ facilitiesAlessandro CostaThomas SurreyCell ServicesElectron MicroscopyFlow CytometryProteomicsStructural BiologyFull details
Caught in the act: structural dynamics of replication origin activation and fork progression Authors (2) Jacob S LewisAlessandro Costa Biochemical Society Transactions Published 5 May 2020 Crick labsAlessandro CostaFull details
A different twist on centromeric chromatin Authors (2) Fabrizio MartinoAlessandro Costa Structure Published 7 January 2020 Crick labsAlessandro CostaFull details
Mechanism of head-to-head MCM double-hexamer formation revealed by cryo-EM Authors (5)Thomas MillerJulia LockeJulia GreiweJohn DiffleyAlessandro Costa Nature Published 20 November 2019 Crick labs/ facilitiesAlessandro CostaJohn DiffleyElectron MicroscopyStructural BiologyFermentationFull details
A combined structural and biochemical approach reveals translocation and stalling of UvrB on the DNA lesion as a mechanism of damage verification in bacterial nucleotide excision repair Authors (10) Marcin Jaciuk Paolo Swuec Vineet Gaur Joanna M Kasprzak Ludovic Renault Mateusz Dobrychłop Shivlee Nirwal Janusz M BujnickiAlessandro Costa Marcin Nowotny DNA Repair Published 1 January 2020 Crick labsAlessandro CostaFull details
Retroviral integration into nucleosomes through DNA looping and sliding along the histone octamer Authors (9) Marcus D Wilson Ludovic Renault Daniel P Maskell Mohamed GhoneimValerie PyeAndrea Nans David S RuedaPeter CherepanovAlessandro Costa Nature Communications Published 13 September 2019 Crick labs/ facilitiesPeter Cherepanov Alessandro CostaStructural BiologyElectron MicroscopyFull details
Molecular basis for ATP-hydrolysis-driven DNA translocation by the CMG helicase of the eukaryotic replisome Authors (12)Patrik EickhoffHazal KoseFabrizio Martino Tatjana Petojevic Ferdos Abid AliJulia Locke[...]Andrea Nans James M Berger Michael R BotchanHasan YardimciAlessandro Costa Cell Reports Published 3 September 2019 Crick labs/ facilitiesAlessandro CostaHasan YardimciStructural BiologyElectron MicroscopyFull details
Structure of DNA-CMG-Pol epsilon elucidates the roles of the non-catalytic polymerase modules in the eukaryotic replisome Authors (12) Panchali Goswami Ferdos Abid Ali Max E Douglas Julia Locke Andrew Purkiss Agnieszka Janska[...] Anne EarlyAndrea Nans Alan MC CheungJohn DiffleyAlessandro Costa Nature Communications Published 29 November 2018 Crick labs/ facilitiesAlessandro CostaJohn DiffleyStructural BiologyElectron MicroscopyFull details
Preparing frozen-hydrated protein-nucleic acid assemblies for high-resolution cryo-EM imaging Authors (3) Panchali Goswami Julia LockeAlessandro Costa Methods in Molecular Biology Published 30 June 2018 Crick labsAlessandro CostaFull details
The mechanism of eukaryotic CMG helicase activation Authors (4) Max E Douglas Ferdos Abid AliAlessandro CostaJohn Diffley Nature Published 8 March 2018 Crick labs/ facilitiesAlessandro CostaJohn DiffleyBioinformatics and BiostatisticsElectron MicroscopyFull details
Cryo-EM structure of a licensed DNA replication origin Authors (7) Ferdos Abid Ali Max E Douglas Julia Locke Valerie E PyeAndrea NansJohn DiffleyAlessandro Costa Nature Communications Published 21 December 2017 Crick labs/ facilitiesPeter Cherepanov Alessandro CostaJohn DiffleyPeter RosenthalElectron MicroscopyStructural BiologyFull details
Mechanism of ubiquitination and deubiquitination in the Fanconi anemia pathway Authors (9) Sylvie van Twest Vincent J Murphy Charlotte Hodson Winnie Tan Paolo Swuec Julienne J O'Rourke Jörg Heierhorst Wayne Crismani Andrew J Deans Molecular Cell Published 9 December 2017 Crick labsAlessandro CostaFull details
The FA core complex contains a homo-dimeric catalytic module for the symmetric mono-ubiquitination of FANCI-FANCD2 Authors (9) Paolo Swuec Ludovic Renault Aaron Borg Fenil Shah Vincent J Murphy Sylvie van TwestBram Snijders Andrew J DeansAlessandro Costa Cell Reports Published 9 December 2017 Crick labs/ facilitiesAlessandro CostaProteomicsFull details
DNA replication and inter-strand crosslink repair: Symmetric activation of dimeric nanomachines? Authors (2) Paolo SwuecAlessandro Costa Biophysical Chemistry Published 10 November 2017 Crick labsAlessandro CostaFull details
Escorting client proteins to the Hsp90 molecular chaperone Authors (2)Patrik EickhoffAlessandro Costa Structure Published 5 July 2017 Crick labsAlessandro CostaFull details
Cdt1 stabilizes an open MCM ring for helicase loading Authors (10) Jordi Frigola Jun He Kerstin Kinkelin Valerie E Pye Ludovic Renault Max E Douglas Dirk RemusPeter CherepanovAlessandro CostaJohn Diffley Nature Communications Published 23 June 2017 Crick labsPeter Cherepanov Alessandro CostaJohn DiffleyFull details
Cryo-electron microscopy of chromatin biology Authors (2) Marcus D WilsonAlessandro Costa Acta crystallographica. Section D, Structural biology Published 1 June 2017 Crick labsAlessandro CostaFull details
The architecture and function of the chromatin replication machinery Authors (2)Thomas MillerAlessandro Costa Current Opinion in Structural Biology Published 15 April 2017 Crick labsAlessandro CostaFull details
CMG-Pol epsilon dynamics suggests a mechanism for the establishment of leading-strand synthesis in the eukaryotic replisome Authors (8) Jin Chuan Zhou Agnieszka Janska Panchali Goswami Ludovic Renault Ferdos Abid Ali Abhay KotechaJohn DiffleyAlessandro Costa Proceedings of the National Academy of Sciences of the United States of America Published 3 April 2017 Crick labsAlessandro CostaJohn DiffleyFull details
A supramolecular assembly mediates lentiviral DNA integration Authors (14)Allison Ballandras-Colas Daniel P Maskell Erik SerraoJulia Locke Paolo Swuec Stefán R Jónsson[...]Ian Taylor Valgerdur Andrésdóttir Alan N EngelmanAlessandro CostaPeter Cherepanov Science Published 6 January 2017 Crick labs/ facilitiesPeter Cherepanov Alessandro CostaIan TaylorElectron MicroscopyStructural BiologyFull details
