Neural circuit model by the Serio lab.

Andrea Serio : Visualising and controlling dynamic processes in complex cultures

Introduction

We use cutting edge microscopy and molecular biology to visualise what's happening inside live cells and pinpoint the molecular bases of neurodegeneration

Modelling metabolic dynamics in astrocyte-neuronal interactions with iPSC-based models of neurodegeneration using quantitative lifetime imaging

We are interested in understanding the contribution of astrocytes to the pathological mechanism in neurodegenerative disorders. In particular, we are trying to understand how astrocytes influence and regulate the function of neuronal populations, by visualising the dynamic fluctuations in ATP, Calcium and mitochondrial function in general. For this project have developed live imaging protocols based on FRET-sensors and optogenetic indicators, visualised with Fluorescence Lifetime Imaging (FLIM) systems, which allow us to take live snapshots of metabolic dynamics in astrocyte and neurons cocultured on our bio-engineered substrates.