Publications

Extraordinary collateral mutagenesis induced by CX-5461

Authors (1)

Simon Boulton

Nature Genetics

Published 1 January 2024

Crick labs

Simon Boulton

Full details

Quantitative, titratable and high-throughput reporter assays to measure DNA double strand break repair activity in cells

Authors (12)

Eeson Rajendra
Diego Grande
Bethany Mason
Daniela Di Marcantonio
Lucy Armstrong
Graeme Hewitt
[...]
Alessandro Galbiati
Simon Boulton
Robert A Heald
Graeme CM Smith
Helen MR Robinson

Nucleic Acids Research

Published 28 February 2024

Crick labs

Simon Boulton

Full details

Negative DNA supercoiling induces genome-wide Cas9 off-target activity

Authors (13)

Matt Newton
Marialucrezia Losito
Quentin M Smith
Venkata Nishita Mohan Parnandi
Benjamin J Taylor
Pinar Akcakaya
[...]
Simon H Reed
Simon Boulton
Graeme A King
Maria Emanuela Cuomo
David S Rueda

Molecular Cell

Published 5 October 2023

Crick labs

Simon Boulton

Full details

Visualization of direct and diffusion-assisted RAD51 nucleation by full-length human BRCA2 protein

Authors (11)

Molecular Cell

Published 17 August 2023

Crick labs

Full details

Structure and function of the RAD51B-RAD51C-RAD51D-XRCC2 tumour suppressor

Authors (10)

Nature

Published 20 July 2023

Crick labs/ facilities

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Integrated transcriptome landscape of ALS identifies genome instability linked to TDP-43 pathology

Authors (14)

Oliver Ziff
Jacob Neeves
Jamie Mitchell
Giulia Tyzack
Carlos Martinez-Ruiz
Raphaelle Luisier
[...]
Simon Boulton
Ammar Al-Chalabi
Gavin Kelly
Jack Humphrey
Rickie Patani

Nature Communications

Published 20 April 2023

Crick labs/ facilities

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The APE2 nuclease is essential for DNA double-strand break repair by microhomology-mediated end joining

Authors (18)

Hubert Fleury
Myles K MacEachern
Clara M Stiefel
Roopesh Anand
Colin Sempeck
Benjamin Nebenfuehr
[...]
Laila Weatherly
Djelika Dansoko
Justin W Leung
Simon Boulton
Nausica Arnoult

Molecular Cell

Published 4 May 2023

Crick labs

Simon Boulton

Full details

Modulating mutational outcomes and improving precise gene editing at CRISPR-Cas9-induced breaks by chemical inhibition of end-joining pathways

Authors (11)

Joost Schimmel
Núria Muñoz-Subirana
Hanneke Kool
Robin van Schendel
Sven van der Vlies
Juliette A Kamp
Femke de Vrij
Steven A Kushner
Graeme CM Smith
Simon Boulton
Marcel Tijsterman

Cell Reports

Published 28 February 2023

Crick labs

Simon Boulton

Full details

Small-molecule Polθ inhibitors provide safe and effective tumor radiosensitization in preclinical models

Authors (24)

Gonzalo Rodriguez-Berriguete
Marco Ranzani
Remko Prevo
Rathi Puliyadi
Nicole Machado
Hannah R Bolland
[...]
Ester M Hammond
Robert A Heald
Graeme CM Smith
Helen MR Robinson
Geoff S Higgins

Clinical cancer research

Published 15 April 2023

Crick labs

Simon Boulton

Full details

POLQ seals post-replicative ssDNA gaps to maintain genome stability in BRCA-deficient cancer cells

Authors (16)

Ondrej Belan
Marie Sebald
Marek Adamowicz
Roopesh Anand
Aleksandra Vancevska
Joana Neves
[...]
Geoff S Higgins
Graeme CM Smith
Stephen West
David S Rueda
Simon Boulton

Molecular Cell

Published 15 December 2022

Crick labs

Full details

Search and processing of Holliday junctions within long DNA by junction-resolving enzymes

Authors (6)

Artur P Kaczmarczyk
Anne-Cécile Déclais
Matt Newton
Simon Boulton
David MJ Lilley
David S Rueda

Nature Communications

Published 7 October 2022

Crick labs

Simon Boulton

Full details

Discovery, characterization, and structure-based optimization of small-molecule in vitro and in vivo probes for human DNA polymerase theta

Authors (33)

Martin L Stockley
Amanda Ferdinand
Giovanni Benedetti
Peter Blencowe
Susan M Boyd
Mat Calder
[...]
Graeme CM Smith
Martin E Swarbrick
Andrew P Turnbull
Simon Willis
Robert A Heald

Journal of Medicinal Chemistry

Published 27 October 2022

Crick labs

Simon Boulton

Full details

Satellite repeat transcripts modulate heterochromatin condensates and safeguard chromosome stability in mouse embryonic stem cells

Authors (13)

Clara Novo
Emily V Wong
Colin Hockings
Chetan Poudel
Eleanor Sheekey
Meike Wiese
[...]
Srinjan Basu
Simon Walker
Gabriele S Kaminski Schierle
Geeta J Narlikar
Peter J Rugg-Gunn

Nature Communications

Published 20 June 2022

Crick labs

Simon Boulton

Full details

Disrupted control of origin activation compromises genome integrity upon destabilization of Polε and dysfunction of the TRP53-CDKN1A/P21 axis

Authors (12)

Valerie Borel
Stefan Boeing
Niek Van Wietmarschen
Sriram Sridharan
Bethany Rebekah Hill
Luigi Ombrato
[...]
Deb Jackson
Robert Goldstone
Simon Boulton
Andre Nussenzweig
Roberto Bellelli

Cell Reports

Published 31 May 2022

Crick labs/ facilities

Full details

H3K4 methylation by SETD1A/BOD1L facilitates RIF1-dependent NHEJ

Authors (14)

Rachel Bayley
Valerie Borel
Rhiannon J Moss
Ellie Sweatman
Phil Ruis
Alice Ormrod
[...]
Graeme Hewitt
Marco Saponaro
Grant S Stewart
Simon Boulton
Martin R Higgs

Molecular Cell

Published 19 May 2022

Crick labs/ facilities

Full details

Proline-specific aminopeptidase P prevents replication-associated genome instability

Authors (10)

Nicola Silva
Maikel Castellano-Pozo
Kenichiro Matsuzaki
Consuelo Barroso
Monica Roman-Trufero
Hannah Craig
Darren R Brooks
R Elwyn Isaac
Simon Boulton
Enrique Martinez-Perez

PLOS Genetics

Published 26 January 2022

Crick labs

Simon Boulton

Full details

HELQ is a dual-function DSB repair enzyme modulated by RPA and RAD51

Authors (10)

Roopesh Anand
Erika Buechelmaier
Ondrej Belan
Matt Newton
Aleksandra Vancevska
Artur Kaczmarczyk
Tohru Takaki
David S Rueda
Simon N Powell
Simon Boulton

Nature

Published 13 January 2022

Crick labs

Simon Boulton

Full details

Tackling PARP inhibitor resistance

Authors (4)

Trends in Cancer

Published 1 December 2021

Crick labs

Full details

Nucleotide proofreading functions by nematode RAD51 paralogs facilitate optimal RAD51 filament function

Authors (5)

Mário Špírek
Martin RG Taylor
Ondrej Belan
Simon Boulton
Lumir Krejci

Nature Communications

Published 20 September 2021

Crick labs

Simon Boulton

Full details

Structure and dynamics of the chromatin remodeler ALC1 bound to a PARylated nucleosome

Authors (10)

Luka Bacic
Guillaume Gaullier
Anton Sabantsev
Laura C Lehmann
Klaus Brackmann
Despoina Dimakou
Mario Halic
Graeme Hewitt
Simon Boulton
Sebastian Deindl

eLife

Published 6 September 2021

Crick labs

Simon Boulton

Full details

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Extraordinary collateral mutagenesis induced by CX-5461

Quantitative, titratable and high-throughput reporter assays to measure DNA double strand break repair activity in cells

Negative DNA supercoiling induces genome-wide Cas9 off-target activity

Visualization of direct and diffusion-assisted RAD51 nucleation by full-length human BRCA2 protein

Structure and function of the RAD51B-RAD51C-RAD51D-XRCC2 tumour suppressor

Integrated transcriptome landscape of ALS identifies genome instability linked to TDP-43 pathology

The APE2 nuclease is essential for DNA double-strand break repair by microhomology-mediated end joining

Modulating mutational outcomes and improving precise gene editing at CRISPR-Cas9-induced breaks by chemical inhibition of end-joining pathways

Small-molecule Polθ inhibitors provide safe and effective tumor radiosensitization in preclinical models

POLQ seals post-replicative ssDNA gaps to maintain genome stability in BRCA-deficient cancer cells

Search and processing of Holliday junctions within long DNA by junction-resolving enzymes

Discovery, characterization, and structure-based optimization of small-molecule in vitro and in vivo probes for human DNA polymerase theta

Satellite repeat transcripts modulate heterochromatin condensates and safeguard chromosome stability in mouse embryonic stem cells

Disrupted control of origin activation compromises genome integrity upon destabilization of Polε and dysfunction of the TRP53-CDKN1A/P21 axis

H3K4 methylation by SETD1A/BOD1L facilitates RIF1-dependent NHEJ

Proline-specific aminopeptidase P prevents replication-associated genome instability

HELQ is a dual-function DSB repair enzyme modulated by RPA and RAD51

Tackling PARP inhibitor resistance

Nucleotide proofreading functions by nematode RAD51 paralogs facilitate optimal RAD51 filament function

Structure and dynamics of the chromatin remodeler ALC1 bound to a PARylated nucleosome

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