Advanced Sequencing : Applications

The Advanced Sequencing team provides high-quality Next Generation Sequence (NGS) data from a state-of-the-art, multi-platform core facility.

We can provide complete project management, from initial planning and sample submission, through library preparation and sequencing, and onward to data analysis, which can be performed by the Bioinformatics STP.

We have expertise in a wide range of NGS applications including RNA-Seq, ChIP-Seq, single cell sequencing (10X Chromium), genomic DNA sequencing and exome capture methods. We are happy to accept pre-made libraries as well as raw samples, all of which undergo an initial, as well as continuous, quality control assessment.

We run multiple short-read Illumina platforms as well as Oxford Nanopore for long read sequencing. We constantly evaluate new technologies and platforms, and automate many sample preparation methods in order to increase throughput.

We have a dedicated Single Cell Suite, suitable for CL2 work. We have implemented Slide-seq for Spatial Transcriptomics.

We mostly perform individual projects designed to address a specific biological question but are also supporting long-term efforts within the Crick:

• Exome and targeted sequencing for the TRACERx consortium
• CRISPR screening (short and long amplicon sequencing) for the Genetic Modification Service (GeMS)
• Karyotyping of cell lines and animals by low-pass sequencing
• Virus sequencing for the Worldwide Influenza Centre
• Setup and operation of the COVID-19 testing pipeline
• SARS-CoV-2 sequencing for COG-UK and the Crick COVID-19 Consortium

We work only with Crick scientists and regretfully cannot accept outside submissions.