Images produced by the light microscopy team at The Crick.

Light Microscopy : Latest news

News and updates about Crick Advanced Light Microscopy STP

Latest CALM updates:

New Confocal Arrivals: Installation of our new LSM 880 multi-photon system will begin the week of June 24th. The 880 MP will replace the 780 MP, which was moved to the 5th floor and remains open for general use as a CALM system. The new 880 MP is also inverted and will have a Ti:Saphire / OPO laser combination that expands imaging of multiple fluorophores through dual-line excitation and wavelength mixing. The following week we will take delivery on a new LSM 880 with AiryScan (also inverted). This workhorse will help take pressure of our existing 880 AiryScan system. Following the release of the new LSM 900 series in April (after we ordered our systems in December...) we are in discussion with Zeiss to upgrade the 880 Airyscan to a 980 in the near future. See Matt or Rocco for details.

Open Staff Positions: After 37 years working for the Crick, CRUK, and the ICRF, Trevor Duhig has moved on from his role as Senior Microscopist. We are currently advertising at the SLRS level for someone to fill Trevor’s position, which will involve a focus on confocal quality control. We also have approval now for a position at the LRS level to help with the management of confocal microscopes outwith the STP. This person will perform quality control checks, help manage repairs, and work closely with Trevor’s replacement to develop a QC strategy for confocal microscopes across the institute. See Kurt for details.

Data management: We now have a test version of OMERO running to evaluate this software for managing image data. Individual labs can use OMERO to facilitate the organisation, analysis, and sharing of image data. It has useful features for both researchers and lab heads. Our project to evaluate OMERO also has work packages dealing with Histopathology, High Throughput Screening, Long-term Time-lapse imaging, and analysis of STORM data using the high-performance cluster. See Kurt or Donald for details.

Huygens for AiryScan: SVI have released a Huygens module for deconvolution of AiryScan data which really improves on Zeiss’ own software. The Huygens software now accounts for the special geometry of the AiryScan detector. Anyone using the AiryScan for super-resolution should strongly consider processing their data using Huygens instead (for which we have a server license). See Dave or Matt for details.

Super-Resolution Demos: We currently have the OMX SR SIM system on extended demo. This system is suitable for imaging live and fixed tissue-culture cells and offers some advantages (as well as some disadvantages...) over the AiryScan. In July we will demo super-resolving spinning disk systems from Nikon and Olympus. See Matt or Donald for details.

Intravital Microscopy: Our new Olympus FV3000 multi-photon system has been installed in LB2-2681. This system offers several improvements over our LSM 700 MP system for intravital microscopy, including better correction for deep tissue imaging, extended laser tuning, and more sensitive detectors. The system is equipped with Olympus’ motorised 25x / 1.05 NA dipping objective which improves signal and resolution in tissue. It also has an Insight X3 laser with extended tuning range from 680 - 1300 nm for excitation of red fluorophores. Finally, the system is equipped with 4 non-descanned PMTs optimised for detection of scattered fluorescence, 2 of which are high-sensitivity GaAsP detectors. We are currently assembling the peripheral kit needed for intravital microscopy (temperature control, anaesthetic unit, dark box) and hope to start training users on this system soon. See Rocco or Donald for details.

Super-Resolution Microscopy: Many people have begun generating useful data with our Vutara STORM microscope. The system is proving easy to use for super-res newbies and has also received positive reviews from more experiences users. Todd Fallesen, our new image analyst, will provide specialised assistance for STORM data analysis. See Deb or Todd for details.

TIRF Microscopy: Our Olympus TIRF system is (finally!) operational. The system is equipped with 4 laser lines which can be individually adjusted for optimal evanescent field illumination (405, 488, 561, and 637 nm). See Donald or Kurt for details.

Training: We have just completed another lecture series on light microscopy, featuring weekly talks from CALM staff on topics including Intro to LM, Optical Sectioning, Confocal, Multi-photon, Light Sheet, and Live Cell Imaging. This series of talks is offered twice per year, and will be offered again starting in October. See Kurt or David Hudson for details.

 

Dr. Kurt I. Anderson
CALM Head
June 2019

Previous CALM updates:

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