Peptide Chemistry STP

Chemical probes

Medicinal chemists at the Crick can offer guidance on best practice in chemical probe synthesis, selection, evaluation and use for testing a biological hypothesis.

If the best published small molecule tool for the planned experimental work is not commercially available, we can help you explore options for obtaining it, including synthesis.

Christelle Soudy offers extensive chemical knowledge and expertise in exploring SciFinder, Reaxys, PubChem and experience accessing MedchemExpress, Chemical Probes.org and Probe Miner

Characteristics of high-quality probes

Characteristics of high quality chemical probes

Pitfalls to be avoided

  • Small molecules are likely to have off target effects because of their small size and low complexity
  • Small molecules which don't dissolve in water, aggregate and react indiscriminately, give misleading results
  • Many drug discovery projects fail at the target validation stage
  • Often 5-10% of compounds in academic screening libraries are PAINS (Pan-assay Interference Compounds)
  • PAINS include toxoflavin, curcumin, resveratrol, rhodanines

Read more about Probeminer and choosing small molecules in Professor Paul Workman's blog post and his Perspective in Cancer Cell, 2017.

Read more about translation resources at the Crick.