α-actinin accounts for the bioactivity of actin preparations in inducing STAT target genes in Drosophila melanogasterMore about Open Access at the Crick
Authors listOliver Gordon Conor Henry Naren Srinivasan Susan Ahrens Anna Franz Safia Deddouche Probir Chakravarty David Phillips Roger George Svend Kjaer David Frith Bram Snijders Rita S Valente Carolina J Simoes da Silva Luis Teixeira Barry Thompson Marc S Dionne Will Wood Caetano Reis e Sousa
Damage-associated molecular patterns (DAMPs) are molecules exposed or released by dead cells that trigger or modulate immunity and tissue repair. In vertebrates, the cytoskeletal component F-actin is a DAMP specifically recognised by DNGR-1, an innate immune receptor. Previously we suggested that actin is also a DAMP in by inducing STAT-dependent genes (<xref ref-type="bibr" rid="bib10">Srinivasan et al., 2016</xref>). Here, we revise that conclusion and report that α-actinin is far more potent than actin at inducing the same STAT response and can be found in trace amounts in actin preparations. Recombinant expression of actin or α-actinin in bacteria demonstrated that only α-actinin could drive the expression of STAT target genes in . The response to injected α-actinin required the same signalling cascade that we had identified in our previous work using actin preparations. Taken together, these data indicate that α-actinin rather than actin drives STAT activation when injected into .