α-actinin accounts for the bioactivity of actin preparations in inducing STAT target genes in Drosophila melanogaster
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Oliver Gordon Conor Henry Naren Srinivasan Susan Ahrens Anna Franz Safia Deddouche Probir Chakravarty David Phillips Roger George Svend Kjaer David Frith Bram Snijders Rita S Valente Carolina J Simoes da Silva Luis Teixeira Barry Thompson Marc S Dionne Will Wood Caetano Reis e SousaAbstract
Damage-associated molecular patterns (DAMPs) are molecules exposed or released by dead cells that trigger or modulate immunity and tissue repair. In vertebrates, the cytoskeletal component F-actin is a DAMP specifically recognised by DNGR-1, an innate immune receptor. Previously we suggested that actin is also a DAMP in by inducing STAT-dependent genes (<xref ref-type="bibr" rid="bib10">Srinivasan et al., 2016</xref>). Here, we revise that conclusion and report that α-actinin is far more potent than actin at inducing the same STAT response and can be found in trace amounts in actin preparations. Recombinant expression of actin or α-actinin in bacteria demonstrated that only α-actinin could drive the expression of STAT target genes in . The response to injected α-actinin required the same signalling cascade that we had identified in our previous work using actin preparations. Taken together, these data indicate that α-actinin rather than actin drives STAT activation when injected into .
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Publisher website (DOI) 10.7554/eLife.38636
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Europe PubMed Central 30260317
Pubmed 30260317
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