A divergent cyclin/cyclin-dependent kinase complex controls the atypical replication of a malaria parasite during gametogony and transmission
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Aurélia C Balestra Mohammad Zeeshan Edward Rea Carla Pasquarello Lorenzo Brusini Tobias Mourier Amit Kumar Subudhi Natacha Klages Patrizia Arboit Rajan Pandey Declan Brady Sue Vaughan Anthony Holder Arnab Pain David JP Ferguson Alexandre Hainard Rita Tewari Mathieu BrochetAbstract
Cell cycle transitions are generally triggered by variation in the activity of cyclin-dependent kinases (CDKs) bound to cyclins. Malaria-causing parasites have a life cycle with unique cell-division cycles, and a repertoire of divergent CDKs and cyclins of poorly understood function and interdependency. We show that Plasmodium berghei CDK-related kinase 5 (CRK5), is a critical regulator of atypical mitosis in the gametogony and is required for mosquito transmission. It phosphorylates canonical CDK motifs of components in the pre-replicative complex and is essential for DNA replication. During a replicative cycle, CRK5 stably interacts with a single Plasmodium-specific cyclin (SOC2), although we obtained no evidence of SOC2 cycling by transcription, translation or degradation. Our results provide evidence that during Plasmodium male gametogony, this divergent cyclin/CDK pair fills the functional space of other eukaryotic cell-cycle kinases controlling DNA replication.
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Publisher website (DOI) 10.7554/eLife.56474
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Europe PubMed Central 32568069
Pubmed 32568069
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