A practical guide to cancer subclonal reconstruction from DNA sequencing

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Abstract

Subclonal reconstruction from bulk tumor DNA sequencing has become a pillar of cancer evolution studies, providing insight into the clonality and relative ordering of mutations and mutational processes. We provide an outline of the complex computational approaches used for subclonal reconstruction from single and multiple tumor samples. We identify the underlying assumptions and uncertainties in each step and suggest best practices for analysis and quality assessment. This guide provides a pragmatic resource for the growing user community of subclonal reconstruction methods.

Journal details

Journal Nature Methods
Volume 18
Issue number 2
Pages 144-155
Available online
Publication date

Crick authors

Crick First author
Crick Corresponding author