A Sanger sequencing protocol for SARS-CoV-2 S-gene
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Rodney S Daniels Ruth Harvey Burcu Ermetal Zheng Xiang Monica Galiano Lorin Adams John MccauleyAbstract
We describe a Sanger sequencing protocol for SARS-CoV-2 S-gene the Spike (S)-glycoprotein product of which, composed of receptor-binding (S1) and membrane fusion (S2) segments, is the target of vaccines used to combat COVID-19. The protocol can be used in laboratories with basic Sanger sequencing capabilities and allows rapid "at source" screening for SARS-CoV-2 variants, notably those of concern. The protocol has been applied for surveillance, with clinical specimens collected in either nucleic acid preservation lysis-mix or virus transport medium, and research involving cultured viruses, and can yield data of public health importance in a timely manner.
Journal details
Volume 15
Issue number 6
Pages 707-710
Available online
Publication date
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Publisher website (DOI) 10.1111/irv.12892
Europe PubMed Central 34346163
Pubmed 34346163