Actin turnover maintains actin filament homeostasis during cytokinetic ring contraction
More about Open Access at the CrickAuthors list
Ting Gang Chew Junqi Huang Saravanan Palani Ruth Sommese Anton Kamnev Tomoyuki Hatano Ying Gu Snezhka Oliferenko Sivaraj Sivaramakrishnan Mohan K BalasubramanianAbstract
Cytokinesis in many eukaryotes involves a tension-generating actomyosin-based contractile ring. Many components of actomyosin rings turn over during contraction, although the significance of this turnover has remained enigmatic. Here, using , we investigate the role of turnover of actin and myosin II in its contraction. Actomyosin ring components self-organize into ∼1-µm-spaced clusters instead of undergoing full-ring contraction in the absence of continuous actin polymerization. This effect is reversed when actin filaments are stabilized. We tested the idea that the function of turnover is to ensure actin filament homeostasis in a synthetic system, in which we abolished turnover by fixing rings in cell ghosts with formaldehyde. We found that these rings contracted fully upon exogenous addition of a vertebrate myosin. We conclude that actin turnover is required to maintain actin filament homeostasis during ring contraction and that the requirement for turnover can be bypassed if homeostasis is achieved artificially.
Journal details
Journal Journal of Cell Biology
Volume 216
Issue number 9
Pages 2657-2667
Available online
Publication date
Full text links
Publisher website (DOI) 10.1083/jcb.201701104
Europe PubMed Central 28655757
Pubmed 28655757
Keywords
Related topics
Type of publication