Altered receptor specificity and cell tropism of D222G haemagglutinin mutants from fatal cases of pandemic A(H1N1) 2009 influenza
Authors list
Yan Liu Robert A Childs Tatyana Matrosovich Stephen Wharton Angelina S Palma Wengang Chai Rodney Daniels Victoria Gregory Jennifer Uhlendorff Makoto Kiso Hans-Dieter Klenk Alan Hay Ten Feizi Mikhail MatrosovichAbstract
Mutations in the receptor-binding site of the hemagglutinin of pandemic influenza A(H1N1) 2009 viruses have been detected sporadically. An Asp222Gly (D222G) substitution has been associated with severe or fatal disease. Here we show that 222G variants infected a higher proportion of ciliated cells in cultures of human airway epithelium than did viruses with 222D or 222E, which targeted mainly nonciliated cells. Carbohydrate microarray analyses showed that 222G variants bind a broader range of α2-3-linked sialyl receptor sequences of a type expressed on ciliated bronchial epithelial cells and on epithelia within the lung. These features of 222G mutants may contribute to exacerbation of disease.
Journal details
Journal Journal of Virology
Volume 84
Issue number 22
Pages 12069-12074
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