An optimized protocol for ChIP-Seq from human embryonic stem cell cultures
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Chromatin immunoprecipitation (ChIP) followed by next-generation sequencing is a powerful technique that characterizes the genome-wide DNA-binding profile of a protein of interest. The general ChIP-seq workflow has been applied widely to many sample types and target proteins, but sample-specific optimization of various steps is necessary to achieve high-quality data. This protocol is specifically optimized for cultured human embryonic stem cells (hESCs), including steps to check sample quality and non-specific enrichment of “hyper-ChIPable” regions prior to sequencing.
Journal details
Journal
STAR Protocols
Volume
1
Issue number
2
Pages
100062
Available online
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Publisher website (DOI)
10.1016/j.xpro.2020.100062
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Europe PubMed Central
33000002
Pubmed
33000002
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