Autophagy modulates endothelial junctions to restrain neutrophil diapedesis during inflammation
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Natalia Reglero-Real Lorena Pérez-Gutiérrez Azumi Yoshimura Loïc Rolas José Garrido-Mesa Anna Barkaway Catherine Pickworth Rebeca S Saleeb Maria Gonzalez-Nuñez Shani N Austin-Williams Dianne Cooper Laura Vázquez-Martínez Tao Fu Giulia De Rossi Matthew Golding Mathieu Benoit-Voisin Chantal M Boulanger Yoshiaki Kubota William A Muller Sharon Tooze Thomas D Nightingale Lucy Collinson Mauro Perretti Ezra Aksoy Sussan NoursharghAbstract
The migration of neutrophils from the blood circulation to sites of infection or injury is a key immune response and requires the breaching of endothelial cells (ECs) that line the inner aspect of blood vessels. Unregulated neutrophil transendothelial cell migration (TEM) is pathogenic, but the molecular basis of its physiological termination remains unknown. Here, we demonstrated that ECs of venules in inflamed tissues exhibited a robust autophagic response that was aligned temporally with the peak of neutrophil trafficking and was strictly localized to EC contacts. Genetic ablation of EC autophagy led to excessive neutrophil TEM and uncontrolled leukocyte migration in murine inflammatory models, while pharmacological induction of autophagy suppressed neutrophil infiltration into tissues. Mechanistically, autophagy regulated the remodeling of EC junctions and expression of key EC adhesion molecules, facilitating their intracellular trafficking and degradation. Collectively, we have identified autophagy as a modulator of EC leukocyte trafficking machinery aimed at terminating physiological inflammation.
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Journal Immunity
Volume 54
Issue number 9
Pages 1989-2004
Available online
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Publisher website (DOI) 10.1016/j.immuni.2021.07.012
Europe PubMed Central 34363750
Pubmed 34363750
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