B cell antigen extraction is regulated by physical properties of antigen-presenting cells
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Antibody production and affinity maturation are driven by B cell extraction and internalization of antigen from immune synapses. However, the extraction mechanism remains poorly understood. Here we develop DNA-based nanosensors to interrogate two previously proposed mechanisms, enzymatic liberation and mechanical force. Using antigens presented by either artificial substrates or live cells, we show that B cells primarily use force-dependent extraction and resort to enzymatic liberation only if mechanical forces fail to retrieve antigen. The use of mechanical forces renders antigen extraction sensitive to the physical properties of the presenting cells. We show that follicular dendritic cells are stiff cells that promote strong B cell pulling forces and stringent affinity discrimination. In contrast, dendritic cells are soft and promote acquisition of low-affinity antigens through low forces. Thus, the mechanical properties of B cell synapses regulate antigen extraction, suggesting that distinct properties of presenting cells support different stages of B cell responses.%U http://jcb.rupress.org/content/jcb/early/2016/12/06/jcb.201607064.full.pdf
Journal details
Journal Journal of Cell Biology
Volume 216
Issue number 1
Pages 217-230
Available online
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Publisher website (DOI) 10.1083/jcb.201607064
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Europe PubMed Central 27923880
Pubmed 27923880
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