B cell-intrinsic requirement for WNK1 kinase in antibody responses in miceMore about Open Access at the Crick
Authors listDarryl Hayward Lesley Vanes Stefanie Wissmann Sujana Sivapatham Harald Hartweger Joshua Biggs O'May Leon De Boer Richard Mitter Robert Koechl Jens V Stein Victor Tybulewicz
Migration and adhesion play critical roles in B cells, regulating recirculation between lymphoid organs, migration within lymphoid tissue, and interaction with CD4+ T cells. However, there is limited knowledge of how B cells integrate chemokine receptor and integrin signaling with B cell activation to generate efficient humoral responses. Here, we show that the WNK1 kinase, a regulator of migration and adhesion, is essential in B cells for T-dependent and -independent antibody responses. We demonstrate that WNK1 transduces signals from the BCR, CXCR5, and CD40, and using intravital imaging, we show that WNK1 regulates migration of naive and activated B cells, and their interactions with T cells. Unexpectedly, we show that WNK1 is required for BCR- and CD40-induced proliferation, acting through the OXSR1 and STK39 kinases, and for efficient B cell-T cell collaboration in vivo. Thus, WNK1 is critical for humoral immune responses, by regulating B cell migration, adhesion, and T cell-dependent activation.
Journal Journal of Experimental Medicine
Issue number 3