Biochemical reconstitution of replication-coupled cohesin acetylation
Abstract
Concomitantly with DNA replication, the cohesin complex establishes cohesion between the newly synthesized sister chromatids. Cohesion establishment requires acetylation of the cohesin subunit Smc3 by the Eco1 acetyltransferase. Acetylation is tightly coupled to DNA replication and depends on (i) DNA replication factors, (ii) DNA structures that transiently form at replication forks, as well as (iii) auxiliary cohesin complex components. Here, we describe a protocol to study DNA replication-coupled cohesin acetylation using purified budding yeast proteins.
Journal details
Journal
Methods in Molecular Biology
Volume
2991
Pages
163-173
Available online
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Publisher website (DOI)
10.1007/978-1-0716-5005-9_11
Europe PubMed Central
41111110
Pubmed
41111110
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