Coronin 1B supports RhoA signaling at cell-cell junctions through Myosin II
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Rashmi Priya Kenneth Wee Srikanth Budnar Guillermo A Gomez Alpha S Yap Magdalene MichaelAbstract
Non-muscle myosin II (NMII) motor proteins are responsible for generating contractile forces inside eukaryotic cells. There is also a growing interest in the capacity for these motor proteins to influence cell signaling through scaffolding, especially in the context of RhoA GTPase signaling. We previously showed that NMIIA accumulation and stability within specific regions of the cell cortex, such as the zonula adherens (ZA), allows the formation of a stable RhoA signaling zone. Now we demonstrate a key role for Coronin 1B in maintaining this junctional pool of NMIIA, as depletion of Coronin 1B significantly compromised myosin accumulation and stability at junctions. The loss of junctional NMIIA, upon Coronin 1B knockdown, perturbed RhoA signaling due to enhanced junctional recruitment of the RhoA antagonist, p190B Rho GAP. This effect was blocked by the expression of phosphomimetic MRLC-DD, thus reinforcing the central role of NMII in regulating RhoA signaling.
Journal details
Journal Cell Cycle
Volume 15
Issue number 22
Pages 3033-3041
Available online
Publication date
Full text links
Publisher website (DOI) 10.1080/15384101.2016.1234549
Europe PubMed Central 27650961
Pubmed 27650961
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