Correlative light electron ion microscopy reveals in vivo localisation of bedaquiline in Mycobacterium tuberculosis-infected lungs
More about Open Access at the CrickAbstract
Correlative light, electron, and ion microscopy (CLEIM) offers huge potential to track the intracellular fate of antibiotics, with organelle-level resolution. However, a correlative approach that enables subcellular antibiotic visualisation in pathogen-infected tissue is lacking. Here, we developed correlative light, electron, and ion microscopy in tissue (CLEIMiT) and used it to identify the cell type-specific accumulation of an antibiotic in lung lesions of mice infected with Mycobacterium tuberculosis. Using CLEIMiT, we found that the anti-tuberculosis (TB) drug bedaquiline (BDQ) is localised not only in foamy macrophages in the lungs during infection but also accumulate in polymorphonuclear (PMN) cells.
Journal details
Journal
PLOS Biology
Volume
18
Issue number
12
Pages
e3000879
Available online
Publication date
Full text links
Publisher website (DOI)
10.1371/journal.pbio.3000879
Figshare
View on figshare
Europe PubMed Central
33382684
Pubmed
33382684
Keywords
Related topics
Type of publication
