Cryogenic OrbiSIMS localizes semi-volatile molecules in biological tissues
Authors listClare Newell Jean-Luc Vorng James MacRae Ian S Gilmore Alex Gould
OrbiSIMS is a recently developed instrument for label-free imaging of chemicals with micron spatial resolution and high mass resolution. We report a cryogenic workflow for OrbiSIMS (Cryo-OrbiSIMS) that improves chemical detection of lipids and other biomolecules in tissues. Cryo-OrbiSIMS boosts ionization yield and decreases ion-beam induced fragmentation, greatly improving the detection of biomolecules such as triacylglycerides. It also increases chemical coverage to include molecules with intermediate or high vapor pressures, such as free fatty acids and semi-volatile organic compounds (SVOCs). We find that Cryo-OrbiSIMS reveals the hitherto unknown localization patterns of SVOCs with high spatial and chemical resolution in diverse plant, animal and human tissues. We also show that Cryo-OrbiSIMS can be combined with genetic analysis to identify enzymes regulating SVOC metabolism. Cryo-OrbiSIMS is applicable to high resolution imaging of a wide variety of non-volatile and semi-volatile molecules across many areas of biomedicine.
Pages Epub ahead of print
Discovery with a purpose - to improve people's health and wellbeing - is central to the Crick.
We conduct discovery science, aimed at solving human health challenges. Our translation and innovation strategy seeks to turn this science into improvements in the lives of people around the world, as well as generating economic opportunities.
We aim to improve lives by identifying possible therapies, diagnosis and disease prevention. This often offers opportunities to form new businesses and grow existing ones, creating highly skilled employment and the potential for other activities that reinforce the UK's position at the forefront of innovation in life sciences.
To realise these health benefits, we have created an environment where basic and clinical scientists can work together closely and effectively.
We don’t carry out patient-based research at The Crick, but we use the clinical and regulatory expertise of our three university founders, their Academic Health Science Centres (AHSCs) and National Institute for Health Research (NIHR) and Biomedical Research Centres (BRCs).
These close working relationships helps in the development of experimental medicine, a key element of translational research.
Our media office helps journalists interested in Crick research and provides media support to our scientists.
Crick logo: go to our image library to download our logo.
Additional image resources will be added to this folder shortly.
+44 (0)20 3796 5252
+44 (0)7918 166 173