Detection of interferon alpha protein reveals differential levels and cellular sources in diseaseMore about Open Access at the Crick
Authors listMathieu P Rodero Jérémie Decalf Vincent Bondet David Hunt Gillian I Rice Scott Werneke Sarah L McGlasson Marie-Alexandra Alyanakian Brigitte Bader-Meunier Christine Barnerias Nathalia Bellon Alexandre Belot Christine Bodemer Tracy A Briggs Isabelle Desguerre Marie-Louise Frémond Marie Hully Arn MJM van den Maagdenberg Isabelle Melki Isabelle Meyts Lucile Musset Nadine Pelzer Pierre Quartier Gisela M Terwindt Joanna Wardlaw Stewart Wiseman Frédéric Rieux-Laucat Yoann Rose Bénédicte Neven Christina Hertel Adrian Hayday Matthew L Albert Flore Rozenberg Yanick J Crow Darragh Duffy
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Type I interferons (IFNs) are essential mediators of antiviral responses. These cytokines have been implicated in the pathogenesis of autoimmunity, most notably systemic lupus erythematosus (SLE), diabetes mellitus, and dermatomyositis, as well as monogenic type I interferonopathies. Despite a fundamental role in health and disease, the direct quantification of type I IFNs has been challenging. Using single-molecule array (Simoa) digital ELISA technology, we recorded attomolar concentrations of IFNα in healthy donors, viral infection, and complex and monogenic interferonopathies. IFNα protein correlated well with functional activity and IFN-stimulated gene expression. High circulating IFNα levels were associated with increased clinical severity in SLE patients, and a study of the cellular source of IFNα protein indicated disease-specific mechanisms. Measurement of IFNα attomolar concentrations by digital ELISA will enhance our understanding of IFN biology and potentially improve the diagnosis and stratification of pathologies associated with IFN dysregulation.
Journal Journal of Experimental Medicine
Issue number 5