Ebola virus VP35 induces high-level production of recombinant TPL-2—ABIN-2—NF-κB1 p105 complex in co-transfected HEK-293 cells
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Thorsten Gantke Sabrina Boussouf Julia Janzen Nicholas A Morrice Steven Howell Elke Mühlberger Steve LeyAbstract
Activation of PKR (double-stranded-RNA-dependent protein kinase) by DNA plasmids decreases translation, and limits the amount of recombinant protein produced by transiently transfected HEK (human embryonic kidney)-293 cells. Co-expression with Ebola virus VP35 (virus protein 35), which blocked plasmid activation of PKR, substantially increased production of recombinant TPL-2 (tumour progression locus 2)-ABIN-2 [A20-binding inhibitor of NF-κB (nuclear factor κB) 2]-NF-κB1 p105 complex. VP35 also increased expression of other co-transfected proteins, suggesting that VP35 could be employed generally to boost recombinant protein production by HEK-293 cells.
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Publisher website (DOI) 10.1042/BJ20121873
Europe PubMed Central 23557442
Pubmed 23557442
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