Engineering orthogonal polypeptide GalNAc-Transferase and UDP-sugar pairs
Authors listJunwon Choi Lauren JS Wagner Suzanne BPE Timmermans Stacy A Malaker Ben Schumann Melissa A Gray Marjoke F Debets Megumi Takashima Jase Gehring Carolyn R Bertozzi
O-Linked α--acetylgalactosamine (O-GalNAc) glycans constitute a major part of the human glycome. They are difficult to study because of the complex interplay of 20 distinct glycosyltransferase isoenzymes that initiate this form of glycosylation, the polypeptide -acetylgalactosaminyltransferases (GalNAc-Ts). Despite proven disease relevance, correlating the activity of individual GalNAc-Ts with biological function remains challenging due to a lack of tools to probe their substrate specificity in a complex biological environment. Here, we develop a "bump-hole" chemical reporter system for studying GalNAc-T activity in vitro. Individual GalNAc-Ts were rationally engineered to contain an enlarged active site (hole) and probed with a newly synthesized collection of 20 (bumped) uridine diphosphate -acetylgalactosamine (UDP-GalNAc) analogs to identify enzymesubstrate pairs that retain peptide specificities but are otherwise completely orthogonal to native enzymesubstrate pairs. The approach was applicable to multiple GalNAc-T isoenzymes, including GalNAc-T1 and -T2 that prefer nonglycosylated peptide substrates and GalNAcT-10 that prefers a preglycosylated peptide substrate. A detailed investigation of enzyme kinetics and specificities revealed the robustness of the approach to faithfully report on GalNAc-T activity and paves the way for studying substrate specificities in living systems.