Exon capture and bulk segregant analysis: rapid discovery of causative mutations using high-throughput sequencing
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Florencia del Viso Dipankan Bhattacharya Yong Kong Michael J Gilchrist Mustafa K KhokhaAbstract
Exome sequencing has transformed human genetic analysis and may do the same for other vertebrate model systems. However, a major challenge is sifting through the large number of sequence variants to identify the causative mutation for a given phenotype. In models like Xenopus tropicalis, an incomplete and occasionally incorrect genome assembly compounds this problem. To facilitate cloning of X. tropicalis mutants identified in forward genetic screens, we sought to combine bulk segregant analysis and exome sequencing into a single step.
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Publisher website (DOI) 10.1186/1471-2164-13-649
Europe PubMed Central 23171430
Pubmed 23171430
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