Expansion of airway basal epithelial cells from primary human non-small cell lung cancer tumors
More about Open Access at the CrickAuthors list
Rob Hynds Assma Ben Aissa Kate HC Gowers Tom Watkins Leticia Bosshard-Carter Andrew Rowan Selvaraju Veeriah Gareth Wilson Sergio A Quezada Charles Swanton TRACERx Consortium Sam M JanesAbstract
Pre-clinical non-small cell lung cancer (NSCLC) models are poorly representative of the considerable inter- and intra-tumor heterogeneity of the disease in patients. Primary cell-based in vitro models of NSCLC are therefore desirable for novel therapy development and personalized cancer medicine. Methods have been described to generate rapidly proliferating epithelial cell cultures from multiple human epithelia using 3T3-J2 feeder cell culture in the presence of Y-27632, a RHO-associated protein kinase (ROCK) inhibitor, in what are known as "conditional reprograming conditions" (CRC) or 3T3 + Y. In some cancer studies, variations of this methodology have allowed primary tumor cell expansion across a number of cancer types but other studies have demonstrated the preferential expansion of normal epithelial cells from tumors in such conditions. Here, we report our experience regarding the derivation of primary NSCLC cell cultures from 12 lung adenocarcinoma patients enrolled in the Tracking Cancer Evolution through Therapy (TRACERx) clinical study and discuss these in the context of improving the success rate for in vitro cultivation of cells from NSCLC tumors.
Journal details
Journal International Journal of Cancer
Volume 143
Issue number 1
Pages 160-166
Available online
Publication date
Full text links
Publisher website (DOI) 10.1002/ijc.31383
Figshare View on figshare
Europe PubMed Central 29569246
Pubmed 29569246