Genetic manipulation of Cryptosporidium parvum with CRISPR/Cas9.

Journal Article: Methods in Molecular BiologyYear Published: (2020) Volume Number: 2052, Article Number: 219-228

Authors

Sateriale,Adam; Pawlowic,Mattie; Vinayak,Sumiti; Brooks,Carrie; Striepen,Boris

Cryptosporidium parvum can be reliably genetically manipulated using CRISPR/Cas9-driven homologous repair coupled to in vivo propagation within immunodeficient mice. Recent modifications have simplified the initial protocol significantly. This chapter will guide through procedures for excystation, transfection, infection, collection, and purification of transgenic Cryptosporidium parvum.