Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition).

Journal Article: European Journal of ImmunologyYear Published: (2019) Volume Number: 49, Article Number: 1457-1973

Authors

Cossarizza,Andrea; Chang,Hyun-Dong; Radbruch,Andreas; Acs,Andreas; Adam,Dieter; Adam-Klages,Sabine; Agace,William W; Aghaeepour,Nima; Akdis,Mübeccel; Allez,Matthieu; Almeida,Larissa Nogueira; Alvisi,Giorgia; Anderson,Graham; Andrä,Immanuel; Annunziato,Francesco; Anselmo,Achille; Bacher,Petra; Baldari,Cosima T; Bari,Sudipto; Barnaba,Vincenzo; Barros-Martins,Joana; Battistini,Luca; Bauer,Wolfgang; Baumgart,Sabine; Baumgarth,Nicole; Baumjohann,Dirk; Baying,Bianka; Bebawy,Mary; Becher,Burkhard; Beisker,Wolfgang; Benes,Vladimir; Beyaert,Rudi; Blanco,Alfonso; Boardman,Dominic A; Bogdan,Christian; Borger,Jessica G; Borsellino,Giovanna; Boulais,Philip E; Bradford,Jolene A; Brenner,Dirk; Brinkman,Ryan R; Brooks,Anna ES; Busch,Dirk H; Büscher,Martin; Bushnell,Timothy P; Calzetti,Federica; Cameron,Garth; Cammarata,Ilenia; Cao,Xuetao; Cardell,Susanna L; Casola,Stefano; Cassatella,Marco A; Cavani,Andrea; Celada,Antonio; Chatenoud,Lucienne; Chattopadhyay,Pratip K; Chow,Sue; Christakou,Eleni; Čičin-Šain,Luka; Clerici,Mario; Colombo,Federico S; Cook,Laura; Cooke,Anne; Cooper,Andrea M; Corbett,Alexandra J; Cosma,Antonio; Cosmi,Lorenzo; Coulie,Pierre G; Cumano,Ana; Cvetkovic,Ljiljana; Dang,Van Duc; Dang-Heine,Chantip; Davey,Martin S; Davies,Derek; De Biasi,Sara; Del Zotto,Genny; Dela Cruz,Gelo Victoriano; Delacher,Michael; Della Bella,Silvia; Dellabona,Paolo; Deniz,Günnur; Dessing,Mark; Di Santo,James P; Diefenbach,Andreas; Dieli,Francesco; Dolf,Andreas; Dörner,Thomas; Dress,Regine J; Dudziak,Diana; Dustin,Michael; Dutertre,Charles-Antoine; Ebner,Friederike; Eckle,Sidonia BG; Edinger,Matthias; Eede,Pascale; Ehrhardt,Götz RA; Eich,Marcus; Engel,Pablo; Engelhardt,Britta; Erdei,Anna; Esser,Charlotte; Everts,Bart; Evrard,Maximilien; Falk,Christine S; Fehniger,Todd A; Felipo-Benavent,Mar; Ferry,Helen; Feuerer,Markus; Filby,Andrew; Filkor,Kata; Fillatreau,Simon; Follo,Marie; Förster,Irmgard; Foster,John; Foulds,Gemma A; Frehse,Britta; Frenette,Paul S; Frischbutter,Stefan; Fritzsche,Wolfgang; Galbraith,David W; Gangaev,Anastasia; Garbi,Natalio; Gaudilliere,Brice; Gazzinelli,Ricardo T; Geginat,Jens; Gerner,Wilhelm; Gherardin,Nicholas A; Ghoreschi,Kamran; Gibellini,Lara; Ginhoux,Florent; Goda,Keisuke; Godfrey,Dale I; Goettlinger,Christoph; González-Navajas,Jose M; Goodyear,Carl S; Gori,Andrea; Grogan,Jane L; Grummitt,Daryl; Grützkau,Andreas; Haftmann,Claudia; Hahn,Jonas; Hammad,Hamida; Hämmerling,Günter; Hansmann,Leo; Hansson,Goran; Harpur,Christopher M; Hartmann,Susanne; Hauser,Andrea; Hauser,Anja E; Haviland,David L; Hedley,David; Hernández,Daniela C; Herrera,Guadalupe; Herrmann,Martin; Hess,Christoph; Höfer,Thomas; Hoffmann,Petra; Hogquist,Kristin; Holland,Tristan; Höllt,Thomas; Holmdahl,Rikard; Hombrink,Pleun; Houston,Jessica P; Hoyer,Bimba F; Huang,Bo; Huang,Fang-Ping; Huber,Johanna E; Huehn,Jochen; Hundemer,Michael; Hunter,Christopher A; Hwang,William YK; Iannone,Anna; Ingelfinger,Florian; Ivison,Sabine M; Jäck,Hans-Martin; Jani,Peter K; Jávega,Beatriz; Jonjic,Stipan; Kaiser,Toralf; Kalina,Tomas; Kamradt,Thomas; Kaufmann,Stefan HE; Keller,Baerbel; Ketelaars,Steven LC; Khalilnezhad,Ahad; Khan,Srijit; Kisielow,Jan; Klenerman,Paul; Knopf,Jasmin; Koay,Hui-Fern; Kobow,Katja; Kolls,Jay K; Kong,Wan Ting; Kopf,Manfred; Korn,Thomas; Kriegsmann,Katharina; Kristyanto,Hendy; Kroneis,Thomas; Krueger,Andreas; Kühne,Jenny; Kukat,Christian; Kunkel,Désirée; Kunze-Schumacher,Heike; Kurosaki,Tomohiro; Kurts,Christian; Kvistborg,Pia; Kwok,Immanuel; Landry,Jonathan; Lantz,Olivier; Lanuti,Paola; LaRosa,Francesca; Lehuen,Agnès; LeibundGut-Landmann,Salomé; Leipold,Michael D; Leung,Leslie YT; Levings,Megan K; Lino,Andreia C; Liotta,Francesco; Litwin,Virginia; Liu,Yanling; Ljunggren,Hans-Gustaf; Lohoff,Michael; Lombardi,Giovanna; Lopez,Lilly; López-Botet,Miguel; Lovett-Racke,Amy E; Lubberts,Erik; Luche,Herve; Ludewig,Burkhard; Lugli,Enrico; Lunemann,Sebastian; Maecker,Holden T; Maggi,Laura; Maguire,Orla; Mair,Florian; Mair,Kerstin H; Mantovani,Alberto; Manz,Rudolf A; Marshall,Aaron J; Martínez-Romero,Alicia; Martrus,Glòria; Marventano,Ivana; Maslinski,Wlodzimierz; Matarese,Giuseppe; Mattioli,Anna Vittoria; Maueröder,Christian; Mazzoni,Alessio; McCluskey,James; McGrath,Mairi; McGuire,Helen M; McInnes,Iain B; Mei,Henrik E; Melchers,Fritz; Melzer,Susanne; Mielenz,Dirk; Miller,Stephen D; Mills,Kingston HG; Minderman,Hans; Mjösberg,Jenny; Moore,Jonni; Moran,Barry; Moretta,Lorenzo; Mosmann,Tim R; Müller,Susann; Multhoff,Gabriele; Muñoz,Luis Enrique; Münz,Christian; Nakayama,Toshinori; Nasi,Milena; Neumann,Katrin; Ng,Lai Guan; Niedobitek,Antonia; Nourshargh,Sussan; Núñez,Gabriel; O'Connor,José-Enrique; Ochel,Aaron; Oja,Anna; Ordonez,Diana; Orfao,Alberto; Orlowski-Oliver,Eva; Ouyang,Wenjun; Oxenius,Annette; Palankar,Raghavendra; Panse,Isabel; Pattanapanyasat,Kovit; Paulsen,Malte; Pavlinic,Dinko; Penter,Livius; Peterson,Pärt; Peth,Christian; Petriz,Jordi; Piancone,Federica; Pickl,Winfried F; Piconese,Silvia; Pinti,Marcello; Pockley,A Graham; Podolska,Malgorzata Justyna; Poon,Zhiyong; Pracht,Katharina; Prinz,Immo; Pucillo,Carlo EM; Quataert,Sally A; Quatrini,Linda; Quinn,Kylie M; Radbruch,Helena; Radstake,Tim RDJ; Rahmig,Susann; Rahn,Hans-Peter; Rajwa,Bartek; Ravichandran,Gevitha; Raz,Yotam; Rebhahn,Jonathan A; Recktenwald,Diether; Reimer,Dorothea; Reis E Sousa,Caetano; Remmerswaal,Ester BM; Richter,Lisa; Rico,Laura G; Riddell,Andy; Rieger,Aja M; Robinson,J Paul; Romagnani,Chiara; Rubartelli,Anna; Ruland,Jürgen; Saalmüller,Armin; Saeys,Yvan; Saito,Takashi; Sakaguchi,Shimon; Sala-de-Oyanguren,Francisco; Samstag,Yvonne; Sanderson,Sharon; Sandrock,Inga; Santoni,Angela; Sanz,Ramon Bellmàs; Saresella,Marina; Sautes-Fridman,Catherine; Sawitzki,Birgit; Schadt,Linda; Scheffold,Alexander; Scherer,Hans U; Schiemann,Matthias; Schildberg,Frank A; Schimisky,Esther; Schlitzer,Andreas; Schlosser,Josephine; Schmid,Stephan; Schmitt,Steffen; Schober,Kilian; Schraivogel,Daniel; Schuh,Wolfgang; Schüler,Thomas; Schulte,Reiner; Schulz,Axel Ronald; Schulz,Sebastian R; Scottá,Cristiano; Scott-Algara,Daniel; Sester,David P; Shankey,T Vincent; Silva-Santos,Bruno; Simon,Anna Katharina; Sitnik,Katarzyna M; Sozzani,Silvano; Speiser,Daniel E; Spidlen,Josef; Stahlberg,Anders; Stall,Alan M; Stanley,Natalie; Stark,Regina; Stehle,Christina; Steinmetz,Tobit; Stockinger,Hannes; Takahama,Yousuke; Takeda,Kiyoshi; Tan,Leonard; Tárnok,Attila; Tiegs,Gisa; Toldi,Gergely; Tornack,Julia; Traggiai,Elisabetta; Trebak,Mohamed; Tree,Timothy IM; Trotter,Joe; Trowsdale,John; Tsoumakidou,Maria; Ulrich,Henning; Urbanczyk,Sophia; van de Veen,Willem; van den Broek,Maries; van der Pol,Edwin; Van Gassen,Sofie; Van Isterdael,Gert; van Lier,René AW; Veldhoen,Marc; Vento-Asturias,Salvador; Vieira,Paulo; Voehringer,David; Volk,Hans-Dieter; von Borstel,Anouk; von Volkmann,Konrad; Waisman,Ari; Walker,Rachael V; Wallace,Paul K; Wang,Sa A; Wang,Xin M; Ward,Michael D; Ward-Hartstonge,Kirsten A; Warnatz,Klaus; Warnes,Gary; Warth,Sarah; Waskow,Claudia; Watson,James V; Watzl,Carsten; Wegener,Leonie; Weisenburger,Thomas; Wiedemann,Annika; Wienands,Jürgen; Wilharm,Anneke; Wilkinson,Robert John; Willimsky,Gerald; Wing,James B; Winkelmann,Rieke; Winkler,Thomas H; Wirz,Oliver F; Wong,Alicia; Wurst,Peter; Yang,Jennie HM; Yang,Juhao; Yazdanbakhsh,Maria; Yu,Liping; Yue,Alice; Zhang,Hanlin; Zhao,Yi; Ziegler,Susanne Maria; Zielinski,Christina; Zimmermann,Jakob; Zychlinsky,Arturo

These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.