High-content analysis of mitochondrial function in iPSC-derived neurons
Abstract
Mitochondrial dysfunction is linked to many neurological diseases; therefore, the ability to measure mitochondrial function is of great use for researching disease and testing potential therapeutics. Here we describe a high-content assay to simultaneously measure mitochondrial membrane potential, morphology and cell viability in iPSC-derived neurons. Neurons are seeded into plates suitable for fluorescent microscopy, stained with the mitochondrial membrane potential-dependent dye TMRM, cytoplasmic dye Calcein AM, and nuclear stain Hoechst 33342. Images are acquired in live cells and analyzed using automated image analysis software.
Journal details
Journal Methods in Molecular Biology
Volume 1994
Pages 175-184
Available online
Publication date
Full text links
Publisher website (DOI) 10.1007/978-1-4939-9477-9_16
Europe PubMed Central 31124115
Pubmed 31124115
Keywords
Type of publication