High-content autophagy analysis in iPSC-derived neurons using immunofluorescence
Abstract
Autophagy is the process by which cellular proteins and organelles are degraded and recycled and is essential to the survival of cells. Defective autophagic degradation has been linked to many neurodegenerative diseases and in particular lysosomal storage diseases. Here we describe a high-content assay to detect defects in the autophagy pathway in induced pluripotent stem cell-derived neurons. This assay utilizes immunofluorescence to stain autophagosomes and uses automated image analysis to measure changes in autophagosome levels in response to modulators of autophagy.
Journal details
Journal Methods in Molecular Biology
Volume 1994
Pages 165-174
Available online
Publication date
Full text links
Publisher website (DOI) 10.1007/978-1-4939-9477-9_15
Europe PubMed Central 31124114
Pubmed 31124114
Keywords
Type of publication