High-speed volumetric imaging of neuronal activity in freely moving rodents
Authors listOliver Skocek Tobias Nöbauer Lukas Weilguny Francisca Martínez Traub Chuying Naomi Xia Maxim Molodtsov Abhinav Grama Masahito Yamagata Daniel Aharoni David D Cox Peyman Golshani Alipasha Vaziri
Thus far, optical recording of neuronal activity in freely behaving animals has been limited to a thin axial range. We present a head-mounted miniaturized light-field microscope (MiniLFM) capable of capturing neuronal network activity within a volume of 700 × 600 × 360 µm at 16 Hz in the hippocampus of freely moving mice. We demonstrate that neurons separated by as little as ~15 µm and at depths up to 360 µm can be discriminated.