High-throughput screening approaches to identify regulators of mammalian autophagy
Abstract
This article discusses the issues to consider in the development and implementation of high-throughput screens (HTSs) using both siRNA libraries and small molecule compound collections, in order to discover autophagy regulators in mammalian cells. We discuss how to develop the screen, focusing on the key parameters to establish in order to perform a successful screen. As our understanding of autophagy increases and its impact on human disease is elucidated, this technology can be further exploited to uncover novel genes, which may one day become new therapeutic targets.
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Publisher website (DOI) 10.1016/j.ymeth.2015.02.002
Europe PubMed Central 25688674
Pubmed 25688674
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