Identification and validation of novel autophagy regulators using an endogenous readout siGENOME screen
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Maria New Tim Van Acker Ming Jiang Rebecca Saunders Jaclyn S Long Jun-Ichi Sakamaki Kevin M Ryan Michael Howell Sharon ToozeAbstract
Autophagy is a highly regulated process, and its deregulation can contribute to various diseases, including cancer, immune diseases, and neurodegenerative disorders. Here we describe the design, protocol, and analysis of an imaging-based high-throughput screen with an endogenous autophagy readout. The screen uses a genome-wide siRNA library to identify autophagy regulators in mammalian cells.
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Publisher website (DOI) 10.1007/978-1-4939-8873-0_24
Europe PubMed Central 30610710
Pubmed 30610710
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