Identification and validation of novel autophagy regulators using an endogenous readout siGENOME screen
Authors listMaria New Tim Van Acker Ming Jiang Rebecca Saunders Jaclyn S Long Jun-Ichi Sakamaki Kevin M Ryan Michael Howell Sharon Tooze
Autophagy is a highly regulated process, and its deregulation can contribute to various diseases, including cancer, immune diseases, and neurodegenerative disorders. Here we describe the design, protocol, and analysis of an imaging-based high-throughput screen with an endogenous autophagy readout. The screen uses a genome-wide siRNA library to identify autophagy regulators in mammalian cells.
Journal Methods in Molecular Biology