Image-based quantitation of host cell-Toxoplasma gondii interplay using HRMAn: A host response to microbe analysis pipeline
Abstract
Research on Toxoplasma gondii and its interplay with the host is often performed using fluorescence microscopy-based imaging experiments combined with manual quantification of acquired images. We present here an accurate and unbiased quantification method for host-pathogen interactions. We describe how to plan experiments and prepare, stain and image infected specimens and analyze them with the program HRMAn (Host Response to Microbe Analysis). HRMAn is a high-content image analysis method based on KNIME Analytics Platform. Users of this guide will be able to perform infection studies in high-throughput volume and to a greater level of detail. Relying on cutting edge machine learning algorithms, HRMAn can be trained and tailored to many experimental settings and questions.
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Journal Methods in Molecular Biology
Volume 2071
Pages 411-433
Available online
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Publisher website (DOI) 10.1007/978-1-4939-9857-9_21
Europe PubMed Central 31758464
Pubmed 31758464
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