Impact of AlphaFold on structure prediction of protein complexes: The CASP15-CAPRI experimentMore about Open Access at the Crick
Authors listMarc F Lensink Guillaume Brysbaert Nessim Raouraoua Paul Bates Marco Giulini Rodrigo V Honorato Charlotte van Noort Joao MC Teixeira Alexandre MJJ Bonvin Ren Kong Hang Shi Xufeng Lu Shan Chang Jian Liu Zhiye Guo Xiao Chen Alex Morehead Raj S Roy Tianqi Wu Nabin Giri Farhan Quadir Chen Chen Jianlin Cheng Carlos A Del Carpio Eichiro Ichiishi Luis A Rodriguez-Lumbreras Juan Fernandez-Recio Ameya Harmalkar Lee-Shin Chu Sam Canner Rituparna Smanta Jeffrey J Gray Hao Li Peicong Lin Jiahua He Huanyu Tao Sheng-You Huang Jorge Roel-Touris Brian Jimenez-Garcia Charles W Christoffer Anika J Jain Yuki Kagaya Harini Kannan Tsukasa Nakamura Genki Terashi Jacob C Verburgt Yuanyuan Zhang Zicong Zhang Hayato Fujuta Masakazu Sekijima Daisuke Kihara Omeir Khan Sergei Kotelnikov Usman Ghani Dzmitry Padhorny Dmitri Beglov Sandor Vajda Dima Kozakov Surendra S Negi Tiziana Ricciardelli Didier Barradas-Bautista Zhen Cao Mohit Chawla Luigi Cavallo Romina Oliva Rui Yin Melyssa Cheung Johnathan D Guest Jessica Lee Brian G Pierce Ben Shor Tomer Cohen Matan Halfon Dina Schneidman-Duhovny Shaowen Zhu Rujie Yin Yuanfei Sun Yang Shen Martyna Maszota-Zieleniak Krzysztof K Bojarski Emilia A Lubecka Mateusz Marcisz Annemarie Danielsson Lukasz Dziadek Margrethe Gaardlos Artur Gieldon Adam Liwo Sergey A Samsonov Rafal Slusarz Karolina Zieba Adam K Sieradzan Cezary Czaplewski Shinpei Kobayashi Yuta Miyakawa Yasuomi Kiyota Mayuko Takeda-Shitaka Kliment Olechnovic Lukas Valancauskas Justas Dapkunas Ceslovas Venclovas Bjorn Wallner Lin Yang Chengyu Hou Xiaodong He Shuai Guo Shenda Jiang Xiaoliang Ma Rui Duan Liming Qui Xianjin Xu Xiaoqin Zou Sameer Velankar Shoshana J Wodak
Toggle all authors (113)
We present the results for CAPRI Round 54, the 5th joint CASP-CAPRI protein assembly prediction challenge. The Round offered 37 targets, including 14 homodimers, 3 homo-trimers, 13 heterodimers including 3 antibody-antigen complexes, and 7 large assemblies. On average ~70 CASP and CAPRI predictor groups, including more than 20 automatics servers, submitted models for each target. A total of 21 941 models submitted by these groups and by 15 CAPRI scorer groups were evaluated using the CAPRI model quality measures and the DockQ score consolidating these measures. The prediction performance was quantified by a weighted score based on the number of models of acceptable quality or higher submitted by each group among their five best models. Results show substantial progress achieved across a significant fraction of the 60+ participating groups. High-quality models were produced for about 40% of the targets compared to 8% two years earlier. This remarkable improvement is due to the wide use of the AlphaFold2 and AlphaFold2-Multimer software and the confidence metrics they provide. Notably, expanded sampling of candidate solutions by manipulating these deep learning inference engines, enriching multiple sequence alignments, or integration of advanced modeling tools, enabled top performing groups to exceed the performance of a standard AlphaFold2-Multimer version used as a yard stick. This notwithstanding, performance remained poor for complexes with antibodies and nanobodies, where evolutionary relationships between the binding partners are lacking, and for complexes featuring conformational flexibility, clearly indicating that the prediction of protein complexes remains a challenging problem.
Issue number 12