In utero electroporation to study mouse brain development.

Journal Article: Methods in Molecular BiologyYear Published: (2020) Volume Number: 2047, Article Number: 513-523

Authors

Pacary,Emilie; Guillemot,François

In utero electroporation is a rapid and powerful technique to study the development of many brain regions. This approach presents several advantages over other methods to study specific steps of brain development in vivo, from proliferation to synaptic integration. Here, we describe in detail the individual steps necessary to carry out the technique. We also highlight the variations that can be implemented to target different cerebral structures and to study specific steps of development.