Insights into the design and interpretation of iCLIP experiments

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Abstract

Ultraviolet (UV) crosslinking and immunoprecipitation (CLIP) identifies the sites on RNAs that are in direct contact with RNA-binding proteins (RBPs). Several variants of CLIP exist, which require different computational approaches for analysis. This variety of approaches can create challenges for a novice user and can hamper insights from multi-study comparisons. Here, we produce data with multiple variants of CLIP and evaluate the data with various computational methods to better understand their suitability.

Journal details

Journal Genome Biology
Volume 18
Issue number 1
Pages 7-7
Available online
Publication date