Insights into the design and interpretation of iCLIP experimentsMore about Open Access at the Crick
Authors listNejc Haberman Ina Huppertz Jan Attig Julian König Zhen Wang Christian Hauer Matthias W Hentze Andreas E Kulozik Hervé Le Hir Tomaž Curk Christopher R Sibley Kathi Zarnack Jernej Ule
Ultraviolet (UV) crosslinking and immunoprecipitation (CLIP) identifies the sites on RNAs that are in direct contact with RNA-binding proteins (RBPs). Several variants of CLIP exist, which require different computational approaches for analysis. This variety of approaches can create challenges for a novice user and can hamper insights from multi-study comparisons. Here, we produce data with multiple variants of CLIP and evaluate the data with various computational methods to better understand their suitability.
Journal Genome Biology
Issue number 1