Loss of NFIX transcription factor biases postnatal neural stem/progenitor cells toward oligodendrogenesis
Authors list
Bo Zhou Jason M Osinski Juan L Mateo Ben Martynoga Fraser J Sim Christine E Campbell Francois Guillemot Michael Piper Richard M GronostajskiAbstract
Murine postnatal neural stem cells (NSCs) give rise to neurons, astrocytes, or oligodendrocytes (OLs); however, our knowledge of the genes that control this lineage specification is incomplete. In this study, we show that nuclear factor I X (NFIX), a transcription factor known to regulate NSC quiescence, also suppresses oligodendrogenesis (ODG) from NSCs. Immunostaining reveals little or no expression of NFIX in OL lineage cells both in vivo and in vitro. Loss of NFIX from subventricular zone (SVZ) NSCs results in enhanced ODG both in vivo and in vitro, while forced expression of NFIX blocks NSC differentiation into OLs in vitro. RNA-seq analysis shows that genes previously shown to be differentially expressed in OL progenitors are significantly enriched in RNA from Nfix(-/-) versus wild-type NSCs. These data indicate that NFIX influences the lineage specification of postnatal SVZ NSCs, specifically suppressing ODG.
Journal details
Journal Stem Cells and Development
Volume 24
Issue number 18
Pages 2114-2126
Available online
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Publisher website (DOI) 10.1089/scd.2015.0136
Europe PubMed Central 26083238
Pubmed 26083238
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