Mapping chromatin features of Xenopus embryos


Chromatin immunoprecipitation (ChIP) combined with genomic analysis provides a global snapshot of protein–DNA interactions in the context of chromatin, yielding insights into which genome loci might be regulated by the DNA-associated protein under investigation. This protocol is an update of a previous version and describes how to perform ChIP on intact or dissected Xenopus embryos. The ChIP-isolated DNA fragments are suitable for both deep sequencing (ChIP-Seq) and quantitative polymerase chain reaction (ChIP-qPCR). General advice for qPCR and for making ChIP-Seq libraries is offered, and approaches for analyzing ChIP-Seq data are outlined.

Journal details

Volume 2019
Issue number 4
Pages pdb.prot100263-pdb.prot100263
Publication date

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