Mechanochemical feedback control of dynamin independent endocytosis modulates membrane tension in adherent cells
Authors list
Joseph Jose Thottacherry Anita Joanna Kosmalska Amit Kumar Amit Singh Vishen Alberto Elosegui Artola Susav Pradhan Sumit Sharma Parvinder P Singh Marta C Guadamillas Natasha Chaudhary Ram Vishwakarma Xavier Trepat Miguel A Del Pozo Robert G Parton Madan Rao Pramod Pullarkat Pere Roca-Cusachs Satyajit MayorAbstract
Plasma membrane tension regulates many key cellular processes. It is modulated by, and can modulate, membrane trafficking. However, the cellular pathway(s) involved in this interplay is poorly understood. Here we find that, among a number of endocytic processes operating simultaneously at the cell surface, a dynamin independent pathway, the CLIC/GEEC (CG) pathway, is rapidly and specifically upregulated upon a sudden reduction of tension. Moreover, inhibition (activation) of the CG pathway results in lower (higher) membrane tension. However, alteration in membrane tension does not directly modulate CG endocytosis. This requires vinculin, a mechano-transducer recruited to focal adhesion in adherent cells. Vinculin acts by controlling the levels of a key regulator of the CG pathway, GBF1, at the plasma membrane. Thus, the CG pathway directly regulates membrane tension and is in turn controlled via a mechano-chemical feedback inhibition, potentially leading to homeostatic regulation of membrane tension in adherent cells.
Journal details
Journal Nature Communications
Volume 9
Issue number 1
Pages 4217-4217
Available online
Publication date
Full text links
Publisher website (DOI) 10.1038/s41467-018-06738-5
Europe PubMed Central 30310066
Pubmed 30310066
Keywords
Type of publication