Monitoring synaptic and neuronal activity in 3D with synthetic and genetic indicators using a compact acousto-optic lens two-photon microscope
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Tomás Fernández-Alfonso KM Naga Srinivas Nadella Florencia Iacaruso Bruno Pichler Hana Roš Paul A Kirkby R Angus SilverAbstract
Two-photon microscopy is widely used to study brain function, but conventional microscopes are too slow to capture the timing of neuronal signalling and imaging is restricted to one plane. Recent development of acousto-optic-deflector-based random access functional imaging has improved the temporal resolution, but the utility of these technologies for mapping 3D synaptic activity patterns and their performance at the excitation wavelengths required to image genetically encoded indicators have not been investigated.
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Publisher website (DOI) 10.1016/j.jneumeth.2013.10.021
Europe PubMed Central 24200507
Pubmed 24200507
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