Multiomic analysis of the UV-induced DNA damage response
More about Open Access at the CrickAuthors list
Stefan Boeing Laura Williamson Vesela Encheva Ilaria Gori Rebecca E Saunders Rachael Instrell Ozan Aygün Marta Rodriguez-Martinez Juston C Weems Gavin Kelly Joan W Conaway Ronald C Conaway Aengus Stewart Michael Howell Bram Snijders Jesper SvejstrupAbstract
In order to facilitate the identification of factors and pathways in the cellular response to UV-induced DNA damage, several descriptive proteomic screens and a functional genomics screen were performed in parallel. Numerous factors could be identified with high confidence when the screen results were superimposed and interpreted together, incorporating biological knowledge. A searchable database, bioLOGIC, which provides access to relevant information about a protein or process of interest, was established to host the results and facilitate data mining. Besides uncovering roles in the DNA damage response for numerous proteins and complexes, including Integrator, Cohesin, PHF3, ASC-1, SCAF4, SCAF8, and SCAF11, we uncovered a role for the poorly studied, melanoma-associated serine/threonine kinase 19 (STK19). Besides effectively uncovering relevant factors, the multiomic approach also provides a systems-wide overview of the diverse cellular processes connected to the transcription-related DNA damage response.
Journal details
Journal Cell Reports
Volume 15
Issue number 7
Pages 1597-1610
Available online
Publication date
Full text links
Publisher website (DOI) 10.1016/j.celrep.2016.04.047
Figshare View on figshare
Europe PubMed Central 27184836
Pubmed 27184836