Nanoscale organization and dynamics of the siglec CD22 cooperate with the cytoskeleton in restraining BCR signallingMore about Open Access at the Crick
Authors listFrancesca Gasparrini Christoph Feest Andreas Bruckbauer Pieta K Mattila Jennifer Müller Lars Nitschke Dennis Bray Facundo D Batista
Receptor organization and dynamics at the cell membrane are important factors of signal transduction regulation. Using super-resolution microscopy and single-particle tracking, we show how the negative coreceptor CD22 works with the cortical cytoskeleton in restraining BCR signalling. In naïve B cells, we found endogenous CD22 to be highly mobile and organized into nanodomains. The landscape of CD22 and its lateral diffusion were perturbed either in the absence of CD45 or when the CD22 lectin domain was mutated. To understand how a relatively low number of CD22 molecules can keep BCR signalling in check, we generated Brownian dynamic simulations and supported them with ex vivo experiments. This combined approach suggests that the inhibitory function of CD22 is influenced by its nanoscale organization and is ensured by its fast diffusion enabling a "global BCR surveillance" at the plasma membrane.
Journal EMBO Journal
Issue number 3