New insights into the mechanism of DNA duplication by the eukaryotic replisome
Abstract
The DNA replication machinery, or replisome, is a macromolecular complex that combines DNA unwinding, priming and synthesis activities. In eukaryotic cells, the helicase and polymerases are multi-subunit, highly-dynamic assemblies whose structural characterization requires an integrated approach. Recent studies have combined single-particle electron cryo-microscopy and protein crystallography to gain insights into the mechanism of DNA duplication by the eukaryotic replisome. We review current understanding of how replication fork unwinding by the CMG helicase is coupled to leading-strand synthesis by polymerase (Pol) ɛ and lagging-strand priming by Pol α/primase, and discuss emerging principles of replisome organization.
Journal details
Journal Trends in Biochemical Sciences
Volume 41
Issue number 10
Pages 859-871
Publication date
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Publisher website (DOI) 10.1016/j.tibs.2016.07.011
Europe PubMed Central 27555051
Pubmed 27555051
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