Normocyte-binding protein required for human erythrocyte invasion by the zoonotic malaria parasite Plasmodium knowlesiMore about Open Access at the Crick
Authors listRobert W Moon Hazem Sharaf Claire H Hastings Yung Shwen Ho Mridul B Nair Zineb Rchiad Ellen Knuepfer Abhinay Ramaprasad Franziska Mohring Amirah Amir Noor A Yusuf Joanna Hall Neil Almond Yee Ling Lau Arnab Pain Michael Blackman Anthony Holder
The dominant cause of malaria in Malaysia is now Plasmodium knowlesi, a zoonotic parasite of cynomolgus macaque monkeys found throughout South East Asia. Comparative genomic analysis of parasites adapted to in vitro growth in either cynomolgus or human RBCs identified a genomic deletion that includes the gene encoding normocyte-binding protein Xa (NBPXa) in parasites growing in cynomolgus RBCs but not in human RBCs. Experimental deletion of the NBPXa gene in parasites adapted to growth in human RBCs (which retain the ability to grow in cynomolgus RBCs) restricted them to cynomolgus RBCs, demonstrating that this gene is selectively required for parasite multiplication and growth in human RBCs. NBPXa-null parasites could bind to human RBCs, but invasion of these cells was severely impaired. Therefore, NBPXa is identified as a key mediator of P. knowlesi human infection and may be a target for vaccine development against this emerging pathogen.
Issue number 26
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Publisher website (DOI) 10.1073/pnas.1522469113
Europe PubMed Central 27303038
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